Results 91 to 100 of about 747,457 (309)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Interrogation in criminal proceedings: pre-trial investigation and trial

Uzhhorod National University Herald. Series: Law
Досліджено процесуальний і тактичний аспекти допиту під час досудового розслідування й судового розгляду. Визначено спільні риси та відмінності допитів, що проводяться на різних стадіях кримінального провадження. Окреслено процесуальні особливості допиту учасників досудового розслідування (потерпілого, свідка, підозрюваного).
Y. M. Myroshnychenko, A. F. Volobuiev
openaire   +3 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Theoretical Basis of Prosecutor's Inspections in Pre-Trial Criminal Proceedings

Pravo: istoriya i sovremennost', 2020
The prosecutor’s inspection of the implementation of the law in pre-trial criminal proceedings is the main form of prosecutorial supervision over the procedural activities of the bodies of preliminary investigation and inquiry. The study aims to form an idea about the procedure for conducting prosecutorial inspections in the pre-trial stages of ...
E. V. Bogatova, O. A. Gracheva
openaire   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Reform and Digitalization of Pre-trial Proceedings in Criminal Proceedings of the Kyrgyz Republic

Bulletin of Science and Practice, 2020
The article is devoted to the peculiarities of changes in the model of criminal proceedings in the Kyrgyz Republic and the definition of ‘criminal proceedings’ is given in a new edition. It has been established that pre-trial proceedings as a stage in the criminal process; begins with the registration of statements and messages and is the initial ...
openaire   +1 more source

Особливості забезпечення права на захист у кримінальному провадженні : наук.-метод. рек. [PDF]

, 2014
Безпалова О. І. Особливості забезпечення права на захист у кримінальному провадженні : наук.-метод. рек. / О. І. Безпалова, К. Л. Бугайчук, І. О. Святокум; МВС України ; Харківський нац. ун-т внутр. справ; Навч.- наук.
Bezpalova, O. I., Bugaychuk, K. L., ORCID: http://orcid.org/0000-0003-2429-5010, ORCID: http://orcid.org/0000-0003-2886-695X, ORCID: http://orcid.org/0000-0003-4374-5494, ResearcherID: I-5612-2016, ResearcherID: I-7047-2016, ResearcherID: J-7221-2016, Sviatokum, I. O., Безпалова, О. І., Бугайчук, К. Л., Навчально-науковий інститут підготовки фахівців кримінальної міліції, Науково-дослідна лабораторія з проблем протидії злочинності, Святокум, І. О.   +13 more
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Discovering Discovery: Non-Party Access to Pretrial Information in the Federal Courts 1938-2006 [PDF]

, 2007
In the modern era, the pretrial process is critical to the disposition of almost all litigation. The vast majority of cases never go to trial. Those which are contested at trial and upon appeal are often decided upon the results of the information gather
Moskowitz, Seymour
core   +2 more sources