Results 171 to 180 of about 4,917 (213)
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Inflammation, 1980
Rabbit prekallikrein (RPK) was purified from rabbit plasma by ion exchange and lectin column chromatography and preparative polyacrylamide gel electrophoresis. A 1500-fold purification was routinely achieved with a final yield of 5-10%. The purified RPK was found to be a glycoprotein with an apparent molecular weight of 88,000.
R J, Ulevitch +2 more
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Rabbit prekallikrein (RPK) was purified from rabbit plasma by ion exchange and lectin column chromatography and preparative polyacrylamide gel electrophoresis. A 1500-fold purification was routinely achieved with a final yield of 5-10%. The purified RPK was found to be a glycoprotein with an apparent molecular weight of 88,000.
R J, Ulevitch +2 more
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Plasma Prekallikrein and Endotoxemia in Liver Cirrhosis
Thrombosis and Haemostasis, 1981SummaryIn liver disease low prekallikrein levels may be found which has been suggested to be due to diminished synthesis. However, it may also be due to endotoxemia accompanying liver disease. To study the last possibility prekallikrein, endotoxins and Normotest were determined in 18 cirrhosis patients.
A C, van Vliet +3 more
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Prekallikrein deficiency in a family of Belgian horses
Journal of the American Veterinary Medical Association, 1990Summary A 7-year-old Belgian stallion hemorrhaged excessively after castration; the hemostatic mechanism was investigated. The horse had normal one-stage prothrombin time and markedly prolonged activated partial thromboplastin time (aptt). Results of intrinsic coagulation factor assays were all normal with the exception of prekallikrein activity, which
R J, Geor +3 more
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Diagnostic ultrasound activates pure prekallikrein
Blood Coagulation & Fibrinolysis, 2012Diagnostic ultrasound activates the contact phase of human coagulation. This has been seen in human blood or plasma or with purified factor 12. The present work aimed to quantify a possibly triggering action of ultrasound on purified prekallikrein, the second of the two main triggers of the intrinsic hemostasis cascade.
Thomas W, Stief, Volker, Klingmüller
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Immunoelectrophoretic studies of prekallikrein in human plasma
Thrombosis Research, 1982Using a rabbit anti-human prekallikrein antibody crossed immunoelectrophoresis (CIE) and Laurell rocket antigen determinations were done in plasma of subjects with Fletcher (prekallikrein, PKA), Fitzgerald (high molecular weight kininogen), Hageman (XII), and PTA (XI) deficiencies as well as in patients with activation of coagulation (intravascular ...
W E, Hathaway +3 more
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Prekallikrein deficiency in a family of miniature horses
American Journal of Veterinary Research, 1986SUMMARY Two sibling miniature horses, a male and a female, had a normal 1-stage prothrombin time and a prolonged activated-partial thromboplastin time (aptt). The addition of as little as 5% of a normal equine plasma pool to the plasma samples of both horses shortened their prolonged aptt to within normal limits.
M A, Turrentine +3 more
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Assay Methods for Prekallikrein and Kininogens and Their Applications
1979Prekallikrein activity in plasma was assayed using a synthetic peptidyl fluorogenic substrate (carbobenzoxy-L-phenylalanyl-L-arginine 4-methylcoumarinyl-7-amide), after activation of prekallikrein by acetone and kaolin. For total kininogen assay, the pretreatment of plasma at pH 2.0 was the best to eliminate bradykinin potentiators and kininase ...
Y, Uchida +9 more
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Determination of prekallikrein in human plasma: optimal conditions for activating prekallikrein.
The Journal of laboratory and clinical medicine, 1978A method for the assay of human plasma prekallikrein in which a chromogenic synthetic tripeptide, PPAN, is used as a substrate for kallikrein is described. The conversion of prekallikrein to kallikrein is achieved by cold activation (0 degrees C) with water-soluble dextran sulfate.
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