Results 71 to 80 of about 9,170,294 (335)
Non-cryo and hypothermic preservations are two available options for short-term storage of living cells. For long-term cell storage, cryopreservation is an essential procedure as it prolongs the storage time, allowing for the transport and testing of ...
Bo Yan +4 more
doaj +1 more source
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
This study selected Abutilon theophrasti Medicus as the research object and optimized the ultrasonic-assisted heat reflux extraction process using response surface methodology to achieve efficient extraction of phenolic acids from its leaves.
Xiaofei Xie +8 more
doaj +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Development and Application of Current Observation Methods
The classification and name of current observation methods are nonstandard used and non-uniform classified in some professional terms. By referring to the national standards, industry norms and relevant literature, combining with the observation ...
Zhengming QIAO +5 more
doaj
Preparation of highly fluorinated polyurethanes [PDF]
New polyurethanes, formed from a reaction of a prepolymer diol and a perfluorinated diisocyanate, are nonflammable and possess high corrosion resistance and good low temperature flexibility.
Rochow, S. E., Stump, E. C., Jr.
core +1 more source
Engineering W-type steady states for three atoms via dissipation in an optical cavity
We propose a scheme for the dissipative preparation of W-type entangled steady-states of three atoms trapped in an optical cavity. The scheme is based on the competition between the decay processes into and out of the target state.
Alharbi +33 more
core +1 more source
Role of oregano and Citrus species-based essential oil preparation for the control of coccidiosis in broiler chickens [PDF]
Francisco Xavier Gordillo Jaramillo +5 more
openalex +1 more source
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source

