Results 71 to 80 of about 1,238,092 (248)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Adaptation Project of the "Roman Hall" in the City Library of Belgrade

Journal of Architectural and Engineering Research
The adaptation project of the “Roman hall” within the City Library of Belgrade, a cultural monument, began in 2015. The project was initiated to enhance this unique space, primarily used for lectures and book promotions.
Ivana Filipović Yorke, Nevenka Novakovic   +1 more
doaj   +1 more source

Analysis of causes of damage to masonry walls in the historic barrack No. B-115 in the former KL Auschwitz II – Birkenau camp [PDF]

MATEC Web of Conferences
The brick barracks in the former German Nazi Concentration and Extermination camp KL Auschwitz II-Birkenau hold historical importance and are protected by law through their inclusion in the register of historical monuments. They are also inscribed on the
Gromysz Krzysztof, Szoblik Łukasz, Drabczyk Zofia, Tanistra-Różanowska Agnieszka   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Platelet rich plasma compared to viscosupplementation in the treatment of knee osteoarthritis: A systematic review and meta‐analysis of randomised controlled trials with 6 month and 12 month follow‐up

Journal of Experimental Orthopaedics
Purpose Platelet rich plasma (PRP) and hyaluronic acid (HA) have been utilised in the last few decades as a conservative treatment for knee osteoarthritis (OA).
Kian Bagheri, Adithya Shekhar, Eric Kwok, Danton Dungy, Susan L. Stewart, Amir A. Jamali   +5 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Development of Predictive Models for Shelf Life of Two Leafy Vegetables Under Different Storage Temperatures

Journal of Food Quality
Postharvest vegetables are still undergoing metabolic processes primarily through respiration. Although modified atmosphere packaging (MAP) can extend the shelf life of vegetables, it is of great significance to accurately predict the shelf life of ...
Tianyu Ren, Binshan Mu, Xinyu Huang, Keyan Wang, Yijing Li, Yangyang Chen, Xiaoyu Wan, Handong Zhao, Jinwang Li, Fengjun Guo   +9 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Fruit packaging with edible chitosan wrappers

Почвознание, агрохимия и екология
With the sharp increase in food safety issues and the lasting effects of the pandemic, consumers are paying more attention to food safety and healthy eating. Plastic waste is a serious environmental problem due to its long degradation period, which is a
Petya Sabeva, Gabor Zsivanovits, Stoil Zhelyazkov   +2 more
doaj   +1 more source