Results 41 to 50 of about 223,556 (265)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Antibacterial and antifungal activities of Platycladus orientalis leaf extract-mediated Fe2O3 and Ce-doped Fe2O3 nanoparticles

Discover Applied Sciences
Green synthesis of nanomaterials harnesses naturally occurring materials, including plant extracts, to offer environmentally friendly alternatives to conventional biomedicine, agriculture, and other field applications. This study explores the green route
Mercy C. Ogwuegbu, Olalekan C. Olatunde, Trust M. Pfukwa, Doctor M. N. Mthiyane, Olaniyi A. Fawole, Damian C. Onwudiwe   +5 more
doaj   +1 more source

Electronic records long term authenticity and integrity demonstration

Knjižnica, 2011
Long term preservation of electronic data requires introduction of specific technology solutions and organizational measures in order to provide stable environment for electronic record preservation.
Helena Halas, Aljoša Jerman Blažič
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Geodynamic Controls on Mantle Differentiation and Preservation of Long‐Term Geochemical Heterogeneity: Focus on the Primitive Undegassed Mantle

Geochemistry, Geophysics, Geosystems
The compositional evolution of the Earth's mantle is the result of mantle differentiation and thermal evolution. Partial melting of mantle materials produces geochemical heterogeneities, allows for degassing and depends on the thermal state of the mantle,
Nicolas Récalde, J. Huw Davies, James Panton, Don Porcelli, Morten Andersen   +4 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Three case studies in virtual preservation. Applying virtual reality to Cultural Heritage

Agathón, 2019
The paper documents three case studies researching the techniques of Virtual Preservation upon various Cultural Heritage sites in order to preserve material aspects that, in the near future, can be irreparably damaged by the impact of climate change ...
Kai Reaver
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Heritage Value Assessment and Landscape Preservation of Traditional Chinese Villages Based on the Daily Lives of Local Residents: A Study of Tangfang Village in China and the UNESCO HUL Approach

Land
Traditional Chinese villages, recognized as national heritage sites, are invaluable cultural assets. Since 2012, efforts have focused on their preservation, though issues remain such as prioritizing authenticity over usability and material over cultural ...
Junjun Li, Jin Wang, Xun Li
doaj   +1 more source