Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Feature binding and error commission. [PDF]
Foerster A +5 more
europepmc +1 more source
A Quantum-Secure Cryptographic Algorithm Integrating Fractals and Prime Numbers
Gerardo Iovane +2 more
openalex +1 more source
Primes obtained concatenating even numbers n with 0 then with n+2 then again with 0 then with n+5
Marius Coman
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Molecular determinants of signal transduction in tropomyosin receptor kinases
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley +1 more source
Congruency effects can interact even when controlling for the reactivation aversion effect: implications for the generality-specificity paradox in hybrid distractor-interference tasks. [PDF]
Dunaway MG, Weissman DH.
europepmc +1 more source
Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner +6 more
wiley +1 more source
Complete mitochondrial genome of the red alga <i>Besa japonica</i> (Gigartinales, Phyllophoraceae) from Korea. [PDF]
Lee SJ, Oak JH, Lee SR.
europepmc +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source

