Results 11 to 20 of about 100,521 (261)
, 2023 HCT116 cells were either transfected with (A-B) empty vector, (C) CMV:3XFLAG-PRL-3, (D) CMV:GFP-PRL-3, or (E) CMV:PRL-3. Cells were blocked with 2% BSA and stained with 1:1000 1 mg/mL nanobody 19 followed by 1:400 Alexa Fluor® 594-AffiniPure Goat Anti ...
K. Martin Chow (772610) +7 more
core +1 more source
, 2023 HCT116 cells were either transfected with (A-B) empty vector, (C) CMV:3XFLAG-PRL-3, (D) CMV:GFP-PRL-3, or (E) CMV:PRL-3. Cells were blocked with 2% BSA and stained with 1:1000 1 mg/mL nanobody 26 followed by 1:400 Alexa Fluor® 594-AffiniPure Goat Anti ...
K. Martin Chow (772610) +7 more
core +1 more source
, 2023 (A) Immobilized metal affinity chromatography purification of 3XFLAG-PRL-3, with an expected size of 25 kDa. FT2, W1-W5 were concentrated together for final protein amounts.
K. Martin Chow (772610) +7 more
core +1 more source
Teksty Drugie, 2021 Having outlined notions of the intimate public sphere (Lauren Berlant), imperial politics of intimacy (Ann Laura Stoler) as well as cultural definitions of intimacy (Eva Illouz, Anthony Giddens, Michael Herzfeld), Siewior presents intimate narratives from the era of the Polish People’s Republic (PRL) in songs, reportage and films which deconstruct the ...
openaire +2 more sources
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL [PDF]
Molecular Endocrinology, 1994 GH4C1 cells increase storage of rat PRL and accumulation of dense core secretory granules when they are treated with 300 nM insulin, 1 nM estradiol, and 10 nM epidermal growth factor. In the experiments reported here, intracellular rat PRL increased from 4% of the total amount produced in 24 h in control cultures to 15% in treated cultures.
J M, Arrandale, P S, Dannies
openaire +3 more sources
Nanobodies are specific for PRL-3 over the other PRL family members.
, 2023 (A) Schematic of the process of generating and isolating anti-PRL-3 nanobodies (Created with BioRender.com). (B) Binding of each histidine-tagged nanobody (NB, at a concentration of 6.22 pmol) to 5 pmol PRL-1, PRL-2, or PRL-3 in 96-well plates.
K. Martin Chow (772610) +7 more
core +1 more source
Inhibition of Prolactin (PRL)-Induced Proliferative Signals in Breast Cancer Cells by a Molecular Mimic of Phosphorylated PRL, S179D-PRL [PDF]
Endocrinology, 2003 Abstract Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals to several ...
Matthew D, Schroeder +3 more
openaire +2 more sources
Dopamine as a Prolactin (PRL) Inhibitor [PDF]
Endocrine Reviews, 2001 Dopamine is a small and relatively simple molecule that fulfills diverse functions. Within the brain, it acts as a classical neurotransmitter whose attenuation or overactivity can result in disorders such as Parkinson's disease and schizophrenia. Major advances in the cloning and characterization of biosynthetic enzymes, transporters, and receptors ...
N, Ben-Jonathan, R, Hnasko
openaire +2 more sources
Kinetics of nanobody binding to PRL-3.
, 2023 Phosphatase of Regenerating Liver-3 (PRL-3) is associated with cancer progression and metastasis. The mechanisms that drive PRL-3’s oncogenic functions are not well understood, partly due to a lack of research tools available to study this protein.
K. Martin Chow (772610) +7 more
core +1 more source