Results 11 to 20 of about 111,764 (305)

PRL Rebuttal

, 2023
This review continues and concludes in arXiv:2302.04853, as linked above.
Frewer, Michael, Khujadze, George
openaire   +2 more sources

Nanobodies selectively immunoprecipitate PRL-3 from HEK293T cell lysate and IP HA-PRL-3 comparatively to commercially available anti-PRL-3 antibodies.

, 2023
(A) Nanobody 19 pulls down 3XFLAG-PRL-3 with minimal to no pulldown of 3XFLAG-PRL-1 or 3XFLAG-PRL-2. Successful nanobody coupling to Dynabeads was verified using an antibody against the C-terminal 6XHis-tag present on each nanobody. (B) Nanobody 19 pulls
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

Anti-PRL-3 nanobodies partially interact with the PRL-3 active site and site of CNNM3 CBS-domain binding.

, 2023
(A) Phosphatase activity of PRL-3 alone or in complex with nanobody 91, against a generic diFMUP substrate. DiFMUP fluoresces when phosphate is released. The graph shows normalized fluorescence to PRL-3 alone, n = 12 ± standard deviation.
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

PRL-3 localization assessed by nanobody 26 and 19 is altered by N-terminal 3XFLAG and GFP tags.

, 2023
(A) Immunofluorescence of HCT116 cells transfected with CMV:3XFLAG-PRL-3, CMV:GFP-PRL-3, CMV:HA-PRL-3, or CMV-PRL-3, as indicated. Cells were stained with anti-PRL-3 nanobody 26 or 19 (HA) followed by an anti-alpaca VHH coupled to Alexa594 secondary ...
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

Solidarity, Community and Ethics in the Context of Engaged Humanities (Contemporary Historical Novels for Young Readers as Examples of Cause Literature)

Annales Universitatis Mariae Curie-Skłodowska. Sectio N, Educatio Nova, 2023
The purpose of the article is to analyze and interpret contemporary Polish novels for young people that portray the image of the People’s Republic of Poland.
Dorota Michułka
doaj   +1 more source

PRL: A probabilistic relational language [PDF]

Machine Learning, 2006
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Lise Getoor, John Grant
openaire   +2 more sources

PRL-3 is differentially localized in colorectal cancer cells when tagged versus untagged using nanobody 19.

, 2023
HCT116 cells were either transfected with (A-B) empty vector, (C) CMV:3XFLAG-PRL-3, (D) CMV:GFP-PRL-3, or (E) CMV:PRL-3. Cells were blocked with 2% BSA and stained with 1:1000 1 mg/mL nanobody 19 followed by 1:400 Alexa Fluor® 594-AffiniPure Goat Anti ...
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

PRL-3 is differentially localized in colorectal cancer cells when tagged versus untagged using nanobody 91.

, 2023
HCT116 cells were either transfected with (A-B) empty vector, (C) CMV:3XFLAG-PRL-3, (D) CMV:GFP-PRL-3, or (E) CMV:PRL-3. Cells were blocked with 2% BSA and stained with 1:1000 1 mg/mL nanobody 26 followed by 1:400 Alexa Fluor® 594-AffiniPure Goat Anti ...
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

PRL: historie intymne

Teksty Drugie, 2021
Having outlined notions of the intimate public sphere (Lauren Berlant), imperial politics of intimacy (Ann Laura Stoler) as well as cultural definitions of intimacy (Eva Illouz, Anthony Giddens, Michael Herzfeld), Siewior presents intimate narratives from the era of the Polish People’s Republic (PRL) in songs, reportage and films which deconstruct the ...
openaire   +2 more sources

Protein purification and immunoprecipitation controls for detecting 3XFLAG-PRL-3, CBS-HA, and nanobody 91-His interactions.

, 2023
(A) Immobilized metal affinity chromatography purification of 3XFLAG-PRL-3, with an expected size of 25 kDa. FT2, W1-W5 were concentrated together for final protein amounts.
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source