A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
Shortest Walk in a Dungeon Graph
Proceedings of the International Florida Artificial Intelligence Research Society ConferenceWith games like No Man’s Sky and Diablo IV, procedural generation of content in games is ever-increasing. Heuristics are needed to assess the goodness of generated content.
Christopher Durham, Chris Alvin
doaj +1 more source
Rapid pulmonary vein isolation utilizing the third-generation laserballoon – The PhoeniX registry
International Journal of Cardiology: Heart & Vasculature, 2020 Background: Balloon-based ablation systems for pulmonary vein isolation (PVI) are providing procedural safety and efficacy as well as favourable clinical outcome. Although second-generation laser balloon (LB2) based PVI has been shown safety and efficacy
Christian-H. Heeger +15 more
doaj +1 more source
A Modular Generative Approach for Realistic River Deltas: When L-Systems and cGANs Meet
IEEE Access, 2022 Procedural terrain generation aims to create topographically coherent landscapes with realistic terrain features. Realistic landscapes of our blue planet are not complete without river deltas; however, there is an insufficient advancement in the ...
Luis Oswaldo Valencia-Rosado +2 more
doaj +1 more source
Hematopoietic (stem) cells—The elixir of life?
FEBS Letters, EarlyView.The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo +4 more
wiley +1 more source
Towards Urban Digital Twins: A Workflow for Procedural Visualization Using Geospatial Data
Remote SensingA key feature for urban digital twins (DTs) is an automatically generated detailed 3D representation of the built and unbuilt environment from aerial imagery, footprints, LiDAR, or a fusion of these.
Sanjay Somanath +5 more
doaj +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Implementation Of L-System In Procedural City Generation Using Java
ComTech, 2010 Article discusses about the design and implementation of procedural content generation using java, especially the generation of virtual city. It is applied by using L-System to generate the elements of the city and also using some images as the base ...
Surya Sujarwo +2 more
doaj +1 more source
Procedural Generation of Grain Orientations using the Wave Function Collapse Algorithm [PDF]
, 2023 G. Magny-Fokam +2 more
openalex +1 more source