In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Production, optimization, and purification of serratiopeptidase from <i>Bacillus siamensis</i> found in marine sediments. [PDF]
Viswan A +7 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Revolutionizing Corrugated Board Production and Optimization with Artificial Intelligence
In the field of corrugated board production and packaging optimization, the advent of Artificial Intelligence (AI) has initiated a paradigm shift. This paper presents a brief analysis of AI's role in revolutionizing both the production of corrugated ...
Tomasz Garbowski
doaj
A streamline-based production optimization method for waterflooding reservoirs. [PDF]
Zhang L +8 more
europepmc +1 more source
Gellan Gum/Alginate Microparticles as Drug Delivery Vehicles: DOE Production Optimization and Drug Delivery. [PDF]
Carrêlo H +3 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Comprehensive Learning-Enhanced Educational Competition Optimizer for Numerical Optimization and Reservoir Production Optimization. [PDF]
Li S, Luo J.
europepmc +1 more source
Modeling a Multi-Parameter Interaction of Geophysical Controls for Production Optimization in Gas Shale Systems. [PDF]
Xu Y, Chang J, Liu X, Hu Z, Duan X.
europepmc +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source

