Results 21 to 30 of about 253,705 (262)

Assembly and disassembly of branched ubiquitin chains

Frontiers in Molecular Biosciences, 2023
Protein ubiquitylation is an essential post-translational modification that regulates nearly all aspects of eukaryotic cell biology. A diverse collection of ubiquitylation signals, including an extensive repertoire of polymeric ubiquitin chains, leads to
Justin B. Gregor, Dantong Xu, Michael E. French, Michael E. French   +3 more
doaj   +1 more source

Proteasome-associated ubiquitin ligase relays target plant hormone-specific transcriptional activators [PDF]

, 2022
The ubiquitin-proteasome system is vital to hormone-mediated developmental and stress responses in plants. Ubiquitin ligases target hormone-specific transcriptional activators (TAs) for degradation, but how TAs are processed by proteasomes remains ...
Grey, Heather, Spoel, Steven H., Mori, Izumi, Wang, Zhishuo, Genschik, Pascal, Nomoto, Mika, Potuschak, Thomas, Mori, Izumi C., Spoel, Steven, Orosa-Puente, Beatriz, Matsuura, Takakazu, Tada, Yasuomi   +11 more
core   +1 more source

Tissue- and developmental stage-specific changes in the subcellular localization of the 26S proteasome in the ovary of Drosophila melanogaster [PDF]

, 2004
The intracellular localization of the 26S proteasome in the different ovarian cell types of Drosophila melanogaster was studied by means of immunofluorescence staining and laser scanning microscopy, with the use of antibodies specific for regulatory ...
Andó, István, Kurucz, Éva, Udvardy, Andor, Gausz, János, Ádám, Géza, Noselli, Stéphane   +5 more
core   +1 more source

The capture proteasome assay (CAPA) to evaluate subtype-specific proteasome inhibitors

Data in Brief, 2015
We recently developed a new assay to measure proteasome activity in vitro (CAPA for capture proteasome assay) [1], based on proteasome capture on an antibody-coated plate.
Nathalie Vigneron, Joanna Abi Habib, Benoît J. Van den Eynde   +2 more
doaj   +1 more source

The proteasome cap RPT5/Rpt5p subunit prevents aggregation of unfolded ricin A chain [PDF]

, 2013
The plant cytotoxin ricin enters mammalian cells by receptor-mediated endocytosis, undergoing retrograde transport to the endoplasmic reticulum (ER) where its catalytic A chain (RTA) is reductively separated from the holotoxin to enter the cytosol and ...
Spooner, Robert A., Roberts, David M, Roberts, Lynne M, Lord, J Michael, Cook, Jonathan P., Pietroni, Paola, Roberts, David M., Lord, Mike, Roberts, L. M. (Lynne M.), Spooner, Robert A, Vasisht, Nishi, Hartmann-Petersen, Rasmus, Cook, Jonathan P   +12 more
core   +1 more source

Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System

Pharmaceutics, 2023
Gold nanoparticles (AuNPs) are widely used in biomedicine due to their remarkable therapeutic applications. However, little is known about their cytotoxic effects on the ubiquitin proteasome system (UPS).
Bashiru Ibrahim, Taiwo Hassan Akere, Swaroop Chakraborty, Eugenia Valsami-Jones, Hanene Ali-Boucetta   +4 more
doaj   +1 more source

Visualizing Proteasome Activity and Intracellular Localization Using Fluorescent Proteins and Activity-Based Probes

Frontiers in Molecular Biosciences, 2019
The proteasome is a multi-catalytic molecular machine that plays a key role in the degradation of many cytoplasmic and nuclear proteins. The proteasome is essential and proteasome malfunction is associated with various disease pathologies.
Sabine Schipper-Krom, Alicia Sanz Sanz, Emma J. van Bodegraven, Dave Speijer, Bogdan I. Florea, Huib Ovaa, Eric A. Reits   +6 more
doaj   +1 more source

Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b

Tumor Biology, 2017
The proteasome beta-4 subunit is required for the assembly of 20S proteasome complex, forming a pivotal component for the ubiquitin–proteasome system. Emerging evidence indicates that proteasome beta-4 subunit may be involved in underlying progression ...
Xiaodong Zhang, Di Lin, Yueqin Lin, Hongqing Chen, Minghua Zou, Shan Zhong, Xuefeng Yi, Siqi Han   +7 more
doaj   +1 more source

High-Throughput/High Content Imaging Screen Identifies Novel Small Molecule Inhibitors and Immunoproteasomes as Therapeutic Targets for Chordoma

Pharmaceutics, 2023
Chordomas account for approximately 1–4% of all malignant bone tumors and 20% of primary tumors of the spinal column. It is a rare disease, with an incidence estimated to be approximately 1 per 1,000,000 people.
Amrendra K. Ajay, Philip Chu, Poojan Patel, Christa Deban, Chitran Roychowdhury, Radhika Heda, Ahmad Halawi, Anis Saad, Nour Younis, Hao Zhang, Xiuju Jiang, Mahmoud Nasr, Li-Li Hsiao, Gang Lin, Jamil R. Azzi   +14 more
doaj   +1 more source

Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the pap1 transcription factor [PDF]

, 2012
Here we report the result of a genetic screen for mutants resistant to the microtubule poison methyl benzimidazol-2-yl carbamate (MBC) that were also temperature sensitive for growth.
Caroline R. Wilkinson (117644), Itaru Samejima, Mathiassen, Søs G, Penney, M., Mary Penney (117638), McInerny Christopher J., Samejima, I., Mairi Wallace (117659), Wallace Mairi, Søs G Mathiassen, Gordon Colin, Gordon, C., Wallace, Mairi, Christopher J. McInerny (117648), Hartmann-Petersen, Rasmus, Christopher J McInerny, McInerny, C.J., McInerny, Christopher J, Mary Penney, Takashi Toda (102488), Rasmus Hartmann-Petersen (117664), Toda, Takashi, Wilkinson, Caroline, Toda, T., Mathiassen Søs G., Penney, Mary, Colin Gordon, Penney Mary, Hartmann-Petersen, R., Itaru Samejima (117642), Wilkinson, C.R., Colin Gordon (117667), Hartmann-Petersen Rasmus, Søs G. Mathiassen (117654), Samejima Itaru, Wilkinson Caroline R., Mathiassen, Søs, Wilkinson, Caroline R, Rasmus Hartmann-Petersen, McInerny, Christopher, Toda Takashi, Wallace, M., Samejima, Itaru, Gordon, Colin, Takashi Toda, Mathiassen, S.G., Caroline R Wilkinson, Mairi Wallace   +47 more
core   +2 more sources