Results 181 to 190 of about 3,609,148 (217)

Role of protein carbonylation in diabetes

Journal of Inherited Metabolic Disease, 2017
AbstractDiabetes mellitus is a metabolic disease characterized by, among others, elevated blood glucose levels. Hyperglycaemia as well as enhanced levels of glucose‐derived reactive metabolites contribute to the development of diabetic complications partly via increased generation of reactive oxygen species (ROS).
Markus, Hecker, Andreas H, Wagner
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Quantification of Protein Carbonylation

2012
Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is most often measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent 2,4 dinitrophenylhydrazine (DNPH).
Nancy B, Wehr, Rodney L, Levine
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Protein Carbonyl Formation in the Diaphragm

American Journal of Respiratory Cell and Molecular Biology, 2005
Abstract Although protein carbonyl formation is an index of oxidative stress in skeletal muscles, the exact proteins, which undergo oxidation in these muscles, remain unknown. We used 2D electrophoresis, immunoblotting, and mass spectrometry to identify carbonylated proteins in the diaphragm in septic animals.
Esther, Barreiro, Joaquim, Gea, Marcos, Di Falco, Leonid, Kriazhev, Susan, James, Sabah N A, Hussain   +5 more
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Investigation of Metal-Catalyzed Antibody Carbonylation With an Improved Protein Carbonylation Assay

Journal of Pharmaceutical Sciences, 2018
Protein carbonylation is a posttranslational modification referring to the occurrence of aldehydes and ketones in proteins. The current understanding of how carbonylation, in particular, metal-catalyzed carbonylation, occurs in recombinant mAbs during production and storage is very limited.
Christian Schöneich, Parbir S Grewal, Inn H Yuk   +2 more
exaly   +3 more sources

Age-related carbonyl stress and erythrocyte membrane protein carbonylation

Clinical Hemorheology and Microcirculation, 2010
Reactive carbonyl species (RCS) have been widely used as indicators of oxidative stress. However, the associations of carbonyl stress with aging process and biochemical alteration of erythrocyte are still poorly understood. Fresh blood samples in vacutainer tubes containing sodium heparinate were obtained from 874 volunteers who were divided into young,
Guolin, Li, Li, Liu, Hui, Hu, Qiong, Zhao, Fuxia, Xie, Keke, Chen, Shenglin, Liu, Yaqin, Chen, Wang, Shi, Dazhong, Yin   +9 more
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Intervention strategies to inhibit protein carbonylation by lipoxidation‐derived reactive carbonyls

Medicinal Research Reviews, 2006
AbstractProtein carbonylation induced by reactive carbonyl species (RCS) generated by peroxidation of polyunsaturated fatty acids plays a significant role in the etiology and/or progression of several human diseases, such as cardiovascular (e.g., atherosclerosis, long‐term complications of diabetes) and neurodegenerative diseases (e.g., Alzheimer's ...
G. Aldini, I. Dalle-Donne, R. Maffei Facino, A. Milzani, M. Carini   +4 more
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Carbonyl Stress: Increased Carbonyl Modification of Tissue and Cellular Proteins in Uremia

Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis, 1999
Advanced glycation end-products (AGEs) are formed during non enzymatic glycation and oxidation (glycoxidation) reactions. This process is accelerated in diabetics owing to hyperglycemia, and it has been implicated in the pathogenesis of diabetic complications. Surprisingly, AGEs increase in normoglycemic uremic patients to a much greater extent than in
T, Miyata, Y, Izuhara, H, Sakai, K, Kurokawa   +3 more
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Protein carbonyl groups in trained subjects

Clinical Hemorheology and Microcirculation, 2012
The purpose of this research was to evaluate the plasma protein carbonyl groups (PC) in 81 trained subjects (TS) who practiced regular, non professional physical activity. They were divided into three groups according to the type of sport they practiced (endurance, mixed or power).
LO PRESTI, Rosalia, CANINO, Baldassare, Montana, M, CALANDRINO, Vincenzo, CAIMI, Gregorio   +4 more
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Profiling Carbonylated Proteins in Human Plasma

Journal of Proteome Research, 2010
This study reports the first proteomic-based identification and characterization of oxidized proteins in human plasma. The study was conducted by isolating carbonylated proteins from the plasma of male subjects (age 32-36) with avidin affinity chromatography subsequent to biotinylation of carbonyl groups with biotin hydrazide and sodium ...
Ashraf G, Madian, Fred E, Regnier
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Determination of Protein Carbonyl Groups by Immunoblotting

Analytical Biochemistry, 1999
Free radical-mediated oxidation of proteins results in the formation of carbonyl groups in quantities that reflect the intensity of the oxidative stress. We have developed an immunochemical technique for the quantification of carbonyl groups in protein samples prepared from small tissue samples and cell cultures.
C E, Robinson, A, Keshavarzian, D S, Pasco, T O, Frommel, D H, Winship, E W, Holmes   +5 more
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