Results 171 to 180 of about 13,056 (198)

Profiling Carbonylated Proteins in Human Plasma

Journal of Proteome Research, 2010
This study reports the first proteomic-based identification and characterization of oxidized proteins in human plasma. The study was conducted by isolating carbonylated proteins from the plasma of male subjects (age 32-36) with avidin affinity chromatography subsequent to biotinylation of carbonyl groups with biotin hydrazide and sodium ...
Ashraf G, Madian, Fred E, Regnier
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Determination of Protein Carbonyl Groups by Immunoblotting

Analytical Biochemistry, 1999
Free radical-mediated oxidation of proteins results in the formation of carbonyl groups in quantities that reflect the intensity of the oxidative stress. We have developed an immunochemical technique for the quantification of carbonyl groups in protein samples prepared from small tissue samples and cell cultures.
C E, Robinson, A, Keshavarzian, D S, Pasco, T O, Frommel, D H, Winship, E W, Holmes   +5 more
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Spectrophotometric Analysis of Protein Carbonyls

2022
Oxidation of proteins is a major threat to their functionality and to the sensory properties and nutritional value of meat and meat products. Moreover, the intake of protein oxidation products may also involve a potential health risk for consumers. The accurate analysis of food protein oxidation seems to be an issue of scientific and technological ...
Francesca Soglia, Giulia Baldi, Alberto Gonzalez-Mohino, Silvia Dıaz-Velasco, Massimiliano Petracci, Mario Estevez   +5 more
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Carbazate modified dextrans as scavengers for carbonylated proteins

Carbohydrate Polymers, 2020
A series of biocompatible and non- toxic polysaccharide molecules have been successfully fabricated and explored their potential application for scavenging the carbonyl species in vitro. These macromolecules were dextrans with different hydrazide substitution ratios determined by TNBS assay, NMR and FTIR characterization.
Bo, Zhou, Ming, Gao, Xianjing, Feng, Lanli, Huang, Quanxin, Huang, Sujit, Kootala, Tobias E, Larsson, Li, Zheng, Tim, Bowden   +8 more
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Protein carbonylation: Proteomics, specificity and relevance to aging

Mass Spectrometry Reviews, 2013
AbstractDetection and quantification of protein carbonyls present in biological samples has become a popular, albeit indirect, method to determine the existence of oxidative stress. Moreover, the rise of proteomics has allowed the identification of the specific proteins targeted by protein carbonylation.
Cabiscol Català, Elisa, Tamarit Sumalla, Jordi, Ros Salvador, Joaquim   +2 more
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Protein carbonyls in meat systems: A review

Meat Science, 2011
Protein oxidation (P-OX) is an innovative topic of increasing interest among meat researchers. Carbonylation is generally recognized as one of the most remarkable chemical modifications in oxidized proteins. In fact, the quantification of protein carbonyls by the dinitrophenylhydrazine (DNPH) method is the most common procedure for assessing P-OX in ...
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A three-dimensional NMR experiment with improved sensitivity for carbonyl–carbonyl J correlation in proteins

Journal of Biomolecular NMR, 1997
Recently, a quantitative J correlation technique has been presented that permits measurement of 3Jc'c' in proteins isotopically enriched with 13C [Hu, J.-S. and Bax, A. (1996) J. Am. Chem. Soc., 118, 8170-8171]. Here, we describe an analogous experiment that is less sensitive to transverse 13C' relaxation, which is the principal limiting factor in all ...
Grzesiek, S., Bax, A.
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Carbonyl-Amine Reactions in Protein Chemistry

1975
Publisher Summary Reaction of a carbonyl group with an amino group is the key reaction in many enzymic and other biological processes, such as vision. This chapter discusses and attempts to organize and correlate the different types of carbonyl–amine reactions found in proteins with their organic chemistry.
R E, Feeney, G, Blankenhorn, H B, Dixon
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Microfluidic Carbonylated Protein Capture

2013
We report a proof of principle study for the use of oxalyldihydrazide as a crosslinker for enrichment of carbonylated proteins within a microfluidic chip. Surface modification steps are characterized and analyzed using analytical techniques. We use in vitro oxidized bovine serum albumin as a model protein for testing the selectivity and specificity of ...
Bryant C. Hollins, Steven A. Soper, June Feng   +2 more
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Effects of carbonylation on the stability of proteins

2009
Proteins frequently become irreversibly modified by carbonylation, a process of introducing the carbonyl group in a reaction with reactive oxygen species (ROS) such as superoxide, peroxide or ozone. Carbonylation increases with the age of cell and is associated with ageing and age related disorders such as Alzheimer's disease, Parkinson's disease and ...
Petrov, Dražen, Hlevnjak, Mario, Zagrovic, Bojan   +2 more
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