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Noninvasive measurement of protein concentration
Magnetic Resonance in Medicine, 1996AbstractAt selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are sensitive to the concentration of rotationally immobilized peptide nitrogen because of field dependent heteronuclear cross relaxation coupling between protein proton and nitrogen‐14 spins that is carried to the water by proton homonuclear cross ...
X, Jiao, R G, Bryant
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Concentrations and partial volumes of proteins
Archives of Biochemistry and Biophysics, 1979Abstract The amount of egg albumin and bovine serum albumin in water has been studied as a function of temperature and time. The temperature selected for heating was 102 °C. The proteins appear to decompose above this temperature. The suitable length of time of drying is 24 h at 102 °C.
H B, Bull, K, Breese
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Spectrophotometric Determination of Protein Concentration
Current Protocols in Protein Science, 2003AbstractThe concentration of a purified protein in solution is most conveniently and accurately measured using absorbance spectroscopy. The absorbance,A, is a linear function of the molar concentration,C, according to the Beer‐Lambert law:A= ɛ ×l×c, where e is the molar absorption coefficient andlis the cell path length.
Gerald R, Grimsley, C Nick, Pace
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Concentration of proteins using carboxymethyldextran
Analytical Biochemistry, 1979Abstract Carboxymethyldextran was used to displace several proteins from DEAE-cellulose in highly concentrated form, and the concentrated protein fractions were examined directly by gel electrophoresis. The concentration of DNA-dependent RNA polymerase II demonstrates the application of the method to a labile enzyme.
A R, Torres, E A, Peterson
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Accurate and Rapid Protein Concentration Measurement of In‐Process, High Concentration Protein Pools
Biotechnology Progress, 2018Protein concentration is a critical product quality attribute and required for any therapeutic protein. Many commercial and investigational new biologics are now formulated at high concentrations (>100 mg/ml) to achieve successful subcutaneous administration.
William S. McKechnie +2 more
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Production and Use of Protein Concentrates
1976The history of utilizing proteins in novel form dates back to gelatin manufacture from hides and bones. In this century the demand for new protein technologies and new sources of raw material first occurred in nonfood areas, such as paper and textile sizings, button manufacture, and even early attempts to make casein fabrics.
Steven R. Tannenbaum, Arthur D. Odell
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Spectrophotometric Determination of Protein Concentration
Current Protocols in Food Analytical Chemistry, 2002AbstractThis unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution. Absorbance measured at 280 nm (A280) is used to calculate protein concentration by comparison with a standard curve or published absorptivity values for that protein (a280).
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Concentrating Proteins for Protein Blotting
2021Protein-related research work often requires solutions containing proteins to be concentrated. Concentrating protein helps improve protein concentration and, with appropriate methods, helps remove unwanted salts or solvents. This chapter presents several available methods to concentrate proteins in the laboratory – all described recently by Dr.
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PROTEIN BINDING AND ANTIBIOTIC CONCENTRATIONS
The Lancet, 1978L R, Peterson, D N, Gerding
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