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Food Science and Nutrition, 2018 This study investigated the effect of groundnut protein concentrate inclusion on the quality of wheat flour. Wheat and groundnut concentrate flours were blended (%, w/w) at ratios 100:0, 95:5, 90:10, 85:15, and 80:20, with 100% wheat flour serving as the
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Dialysis and Concentration of Protein Solutions
Current Protocols in Immunology, 1997AbstractConventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. This appendix describes dialysis of large‐ and small‐volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight.
Sarah M, Andrew +2 more
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Airborne concentrations of peanut protein
Allergy and Asthma Proceedings, 2013Food allergy to peanut is a significant health problem, and there are reported allergic reactions to peanuts despite not eating or having physical contact with peanuts. It is presumed that an allergic reaction may have occurred from inhalation of airborne peanut allergens. The purpose of this study was to detect the possible concentrations of airborne
Rodney M, Johnson, Charles S, Barnes
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Noninvasive measurement of protein concentration
Magnetic Resonance in Medicine, 1996AbstractAt selected magnetic field strengths, protein and water proton spin‐lattice relaxation rates are sensitive to the concentration of rotationally immobilized peptide nitrogen because of field dependent heteronuclear cross relaxation coupling between protein proton and nitrogen‐14 spins that is carried to the water by proton homonuclear cross ...
X, Jiao, R G, Bryant
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Concentrations and partial volumes of proteins
Archives of Biochemistry and Biophysics, 1979Abstract The amount of egg albumin and bovine serum albumin in water has been studied as a function of temperature and time. The temperature selected for heating was 102 °C. The proteins appear to decompose above this temperature. The suitable length of time of drying is 24 h at 102 °C.
H B, Bull, K, Breese
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Concentration of proteins using carboxymethyldextran
Analytical Biochemistry, 1979Abstract Carboxymethyldextran was used to displace several proteins from DEAE-cellulose in highly concentrated form, and the concentrated protein fractions were examined directly by gel electrophoresis. The concentration of DNA-dependent RNA polymerase II demonstrates the application of the method to a labile enzyme.
A R, Torres, E A, Peterson
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Spectrophotometric Determination of Protein Concentration
Current Protocols in Protein Science, 2003AbstractThe concentration of a purified protein in solution is most conveniently and accurately measured using absorbance spectroscopy. The absorbance,A, is a linear function of the molar concentration,C, according to the Beer‐Lambert law:A= ɛ ×l×c, where e is the molar absorption coefficient andlis the cell path length.
Gerald R, Grimsley, C Nick, Pace
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Accurate and Rapid Protein Concentration Measurement of In‐Process, High Concentration Protein Pools
Biotechnology Progress, 2018Protein concentration is a critical product quality attribute and required for any therapeutic protein. Many commercial and investigational new biologics are now formulated at high concentrations (>100 mg/ml) to achieve successful subcutaneous administration.
William S. McKechnie +2 more
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Production and Use of Protein Concentrates
1976The history of utilizing proteins in novel form dates back to gelatin manufacture from hides and bones. In this century the demand for new protein technologies and new sources of raw material first occurred in nonfood areas, such as paper and textile sizings, button manufacture, and even early attempts to make casein fabrics.
Steven R. Tannenbaum, Arthur D. Odell
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Spectrophotometric Determination of Protein Concentration
Current Protocols in Food Analytical Chemistry, 2002AbstractThis unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution. Absorbance measured at 280 nm (A280) is used to calculate protein concentration by comparison with a standard curve or published absorptivity values for that protein (a280).
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