Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates [PDF]
, 2018 Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein's native conformation, which ultimately results in its aggregation or ...
Mata Cabana, Alejandro +3 more
core +2 more sources
Conformational Plasticity of HLA-B27 Molecules Correlates Inversely With Efficiency of Negative T Cell Selection [PDF]
, 2020 The development of autoimmune disorders is incompletely understood. Inefficient thymic T cell selection against self-peptides presented by major histocompatibility antigens (HLA in humans) may contribute to the emergence of auto-reactive effector cells ...
Loll, Bernhard +3 more
core +1 more source
Combining Coarse-Grained Protein Models with Replica-Exchange All-Atom Molecular Dynamics [PDF]
, 2013 We describe a combination of all-atom simulations with CABS, a well-established coarse-grained protein modeling tool, into a single multiscale protocol. The simulation method has been tested on the C-terminal beta hairpin of protein G, a model system of ...
Gront, Dominik +4 more
core +3 more sources
Mobility Measurements Probe Conformational Changes in Membrane Proteins due to Tension
, 2015 The function of membrane-embedded proteins such as ion channels depends crucially on their conformation. We demonstrate how conformational changes in asymmetric membrane proteins may be inferred from measurements of their diffusion.
Morris, Richard G., Turner, Matthew S.
core +1 more source
Focused conformational sampling in proteins [PDF]
The Journal of Chemical Physics, 2017 A detailed understanding of the conformational dynamics of biological molecules is difficult to obtain by experimental techniques due to resolution limitations in both time and space. Computer simulations avoid these in theory but are often too short to sample rare events reliably.
Bacci, Marco +4 more
openaire +4 more sources
pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4 [PDF]
, 2009 Nitrophorins are NO carrier proteins that transport and release NO through a pH-dependent conformational change. They bind NO tightly in a low pH environment and release it in a higher pH environment.
Estrin, Dario Ariel +5 more
core +1 more source
Structure of the atypical bacteriocin pectocin M2 implies a novel mechanism of protein uptake [PDF]
, 2014 The colicin-like bacteriocins are potent protein antibiotics that have evolved to efficiently cross the outer membrane of Gram-negative bacteria by parasitizing nutrient uptake systems.
Adams +50 more
core +3 more sources
CLP-based protein fragment assembly
, 2010 The paper investigates a novel approach, based on Constraint Logic Programming (CLP), to predict the 3D conformation of a protein via fragments assembly. The fragments are extracted by a preprocessor-also developed for this work- from a database of known
AGOSTINO DOVIER +6 more
core +1 more source
Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD [PDF]
, 2018 The dihydroorotase (DHOase) domain of the multifunctional protein carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase (CAD) catalyzes the third step in the de novo biosynthesis of pyrimidine nucleotides in animals.
D'Abramo, Marco +4 more
core +1 more source
Therapeutic Apheresis and Dialysis, EarlyView.ABSTRACT Introduction Pre‐dilution online hemodiafiltration (Pre‐HDF) is predominantly used in Japan, whereas post‐dilution online HDF (Post‐HDF) is more common in Europe. An asymmetric cellulose triacetate (ATA) membrane may improve biocompatibility.
Kenji Sakurai +4 more
wiley +1 more source