Results 31 to 40 of about 103,093 (251)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

The Maturation Pathway of Nickel Urease

Inorganics, 2019
Maturation of urease involves post-translational insertion of nickel ions to form an active site with a carbamylated lysine ligand and is assisted by urease accessory proteins UreD, UreE, UreF and UreG.
Yap Shing Nim, Kam-Bo Wong
doaj   +1 more source

Expression and Characterization of Relaxin Family Peptide Receptor 1 Variants

Frontiers in Pharmacology, 2022
G-protein coupled receptors (GPCR) transduce extracellular stimuli into the cell interior and are thus centrally involved in almost all physiological-neuronal processes.
David Speck, Gunnar Kleinau, Mark Meininghaus, Antje Erbe, Antje Erbe, Alexandra Einfeldt, Alexandra Einfeldt, Michal Szczepek, Patrick Scheerer, Patrick Scheerer, Vera Pütter, Vera Pütter   +11 more
doaj   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Crystal structure of pectocin M1 reveals diverse conformations and interactions during its initial step via the ferredoxin uptake system

FEBS Open Bio
Pectocin M1 (PM1), the bacteriocin from phytopathogenic Pectobacterium carotovorum which causes soft rot disease, has a unique ferredoxin domain that allows it to use FusA of the plant ferredoxin uptake system.
Nawee Jantarit, Hideaki Tanaka, Yuxi Lin, Young‐Ho Lee, Genji Kurisu   +4 more
doaj   +1 more source

Angiotensin and Endothelin Receptor Structures With Implications for Signaling Regulation and Pharmacological Targeting

Frontiers in Endocrinology, 2022
In conjunction with the endothelin (ET) type A (ETAR) and type B (ETBR) receptors, angiotensin (AT) type 1 (AT1R) and type 2 (AT2R) receptors, are peptide-binding class A G-protein-coupled receptors (GPCRs) acting in a physiologically overlapping context.
David Speck, Gunnar Kleinau, Michal Szczepek, Dennis Kwiatkowski, Rusan Catar, Aurélie Philippe, Aurélie Philippe, Patrick Scheerer, Patrick Scheerer   +8 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio
The SARS‐CoV‐2 Nucleocapsid (N) protein plays a crucial role in genome packaging, replication, transcription, and pathogenesis, making it a promising target for antiviral drug development.
Shweta Singh, Gagan D. Gupta
doaj   +1 more source

Insight into the Internal Structure of Biogenic, Synthetic and Geological Apatite by Electron Microscopy and X‐Ray Scattering

Advanced Functional Materials, EarlyView.
Apatite occurs in many forms in nature, e.g. in teeth and geological minerals. Internally, biological apatite contains nanocrystals that are also found in synthetically prepared calcium phosphate nanoparticles which are used in biomedicine, e.g. for gene and drug delivery and for bone regeneration. Abstract Calcium phosphate is the inorganic component (
Kathrin Kostka, Oleg Prymak, Kateryna Loza, Matthias Epple   +3 more
wiley   +1 more source

Structural vulnerability in EPCR suggests functional modulation

Scientific Reports
The endothelial protein C receptor (EPCR) is a fundamental component of the vascular system in mammals due to its contribution in maintaining blood in a non-prothrombotic state, which is crucial for overall life development.
Elena Erausquin, Adela Rodríguez-Fernández, Luis Ángel Rodríguez-Lumbreras, Juan Fernández-Recio, María Gilda Dichiara-Rodríguez, Jacinto López-Sagaseta   +5 more
doaj   +1 more source