Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro [PDF]
International Journal of Inflammation, 2021 Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. The present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation.
Douglas Bosco Aidoo +3 more
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Mechanisms of Change in Emulsifying Capacity Induced by Protein Denaturation and Aggregation in Quick-Frozen Pork Patties with Different Fat Levels and Freeze–Thaw Cycles [PDF]
Foods, 2021 Herein, we discuss changes in the emulsifying properties of myofibrillar protein (MP) because of protein denaturation and aggregation from quick-frozen pork patties with multiple fat levels and freeze–thaw (F–T) cycles.
Nan Pan +7 more
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Protein denaturation at the air-water interface and how to prevent it [PDF]
eLife, 2019 Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in unsupported films of aqueous solution, which can result in partial or complete denaturation. We
Edoardo D'Imprima +5 more
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Comparison of Characterization Methods for Denaturation Degree of Shrimp Head Protein during Heating
Shipin gongye ke-ji, 2022 In order to select the suitable characterization method for the denaturation degree of shrimp head protein, the mixed protein extracted from shrimp head was heated at 100 ℃ for a certain time.
Qiumei LIU +9 more
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Protein stability [determination] problems
Frontiers in Molecular Biosciences, 2022 Human health depends on the correct folding of proteins, for misfolding and aggregation lead to diseases. An unfolded (denatured) protein can refold to its original folded state. How does this occur is known as the protein folding problem. One of several
Faizan Ahmad
doaj +1 more source
Irreversible denaturation of maltodextrin glucosidase studied by differential scanning calorimetry, circular dichroism, and turbidity measurements. [PDF]
PLoS ONE, 2014 Thermal denaturation of Escherichia coli maltodextrin glucosidase was studied by differential scanning calorimetry, circular dichroism (230 nm), and UV-absorption measurements (340 nm), which were respectively used to monitor heat absorption ...
Megha Goyal +2 more
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Highly anomalous energetics of protein cold denaturation linked to folding-unfolding kinetics. [PDF]
PLoS ONE, 2011 Despite several careful experimental analyses, it is not yet clear whether protein cold-denaturation is just a "mirror image" of heat denaturation or whether it shows unique structural and energetic features. Here we report that, for a well-characterized
M Luisa Romero-Romero +3 more
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Protein Unfolding: Denaturant vs. Force [PDF]
Biomedicines, 2021 While protein refolding has been studied for over 50 years since the pioneering work of Christian Anfinsen, there have been a limited number of studies correlating results between chemical, thermal, and mechanical unfolding. The limited knowledge of the relationship between these processes makes it challenging to compare results between studies if ...
Kelly, Colleen, Gage, Matthew J.
openaire +3 more sources
Native protein denaturation using urea
BioTechniques, 2017 Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar +2 more
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Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]
PLoS ONE, 2013 UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva +10 more
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