Results 51 to 60 of about 414,168 (349)

Effect of mechanical denaturation on surface free energy of protein powders [PDF]

, 2016
yesGlobular proteins are important both as therapeutic agents and excipients. However, their fragile native conformations can be denatured during pharmaceutical processing, which leads to modification of the surface energy of their powders and hence ...
Blagbrough, I.S., Conway, B.R., Forbes, Robert T., Grimsey, Ian M., Mohammad, Mohammad A.   +4 more
core   +2 more sources

Effects of Ionic Liquids on Metalloproteins

Molecules, 2021
In the past decade, innovative protein therapies and bio-similar industries have grown rapidly. Additionally, ionic liquids (ILs) have been an area of great interest and rapid development in industrial processes over a similar timeline.
Aashka Y. Patel, Keertana S. Jonnalagadda, Nicholas Paradis, Timothy D. Vaden, Chun Wu, Gregory A. Caputo   +5 more
doaj   +1 more source

Matrix metalloproteinase activities and their relationship with collagen remodelling in tendon pathology [PDF]

, 2002
Our aim was to correlate the activity of matrix metalloproteinases (MMPs) with denaturation and the turnover of collagen in normal and pathological human tendons.
Bank, Ruud A., Curry, Valerie, DeGroot, Jeroen, Hazleman, Brian L., Riley, Graham P., van El, Benno, Verzijl, Nicole   +6 more
core   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

New Powerful Protein Denaturant [PDF]

Biophysical Journal, 2013
Traditional denaturants such as urea and guanidinium hydrochloride effectively unfold a variety of proteins in an “all-or-none” fashion. However their high working concentration in combination with the strong absorption below 210 nm make it impossible to measure high quality circular dichroism spectra, which are commonly used to detect changes in ...
Tatyana Perlova, Hannah Gelman, Martin Gruebele   +2 more
openaire   +2 more sources

Protein sterilization method of firefly luciferase using reduced pressure and molecular sieves [PDF]

, 1973
The sterilization of the protein fruitfly luciferase under conditions that prevent denaturation is examined. Denaturation is prevented by heating the protein in contact with molecular seives and under a reduced pressure of the order of 0.00005 ...
Chappelle, E. W., Rich, E., Jr.
core   +1 more source

Mycobacterium tuberculosis sulfurtransferase SseA is activated by its neighboring gene product Rv3284

FEBS Letters, EarlyView.
Tuberculosis remains a global health challenge and new therapeutic targets are required. Here, we characterized SseA, a sulfurtransferase from Mycobacterium tuberculosis involved in macrophage infection, and its interaction with the newly identified protein SufEMtb that activates SseA enzymatic activity.
Giulia Di Napoli, Alex Fissore, Edoardo Salladini, Eleonora Raccuia, Simonetta Oliaro‐Bosso, Alessia Ruggiero, Rita Berisio, Milagros Medina, Adrian Velazquez‐Campoy, Salvatore Adinolfi, Mauro Marengo   +10 more
wiley   +1 more source

Numerical Simulation of Protein Denaturation of Scallop Adductors during Heating Based on Temperature Distribution and Water Status Distribution

Shipin gongye ke-ji
Protein denaturation is the key point affecting quality attributes of the scallop adductors (SA) during thermal processing such as drying, sous-vide cooking and traditional cooking.
Qilong SHI, Jing LIU, Ya ZHAO
doaj   +1 more source

Visualization of early events in acetic acid denaturation of HIV-1 protease: a molecular dynamics study.

PLoS ONE, 2011
Protein denaturation plays a crucial role in cellular processes. In this study, denaturation of HIV-1 Protease (PR) was investigated by all-atom MD simulations in explicit solvent.
Aditi Narendra Borkar, Aditi Narendra Borkar, Manoj Kumar Rout, Ramakrishna V Hosur   +3 more
doaj   +1 more source

Chemical shifts in denatured proteins: resonance assignments for denatured ubiquitin and comparisons with other denatured proteins.

Journal of biomolecular NMR, 2001
Chemical shift assignment is reported for the protein ubiquitin denatured in 8M urea at pH 2. The variations in 15N chemical shifts of three different proteins (ubiquitin, disulfide reduced, carboxymethylated lysozyme, all-Ala-alpha-lactalbumin), all without disulfides and denatured in 8M urea at pH 2 are compared to 'random coil shifts' of small model
Peti, W, Smith, L, Redfield, C, Schwalbe, H   +3 more
openaire   +4 more sources