Results 81 to 90 of about 414,168 (349)
FEBS Open Bio, EarlyView.In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna +4 more
wiley +1 more source
Hyperthermophile protein behavior: partially-structured conformations of Pyrococcus furiosus rubredoxin monomers generated through forced cold-denaturation and refolding. [PDF]
PLoS ONE, 2014 Some years ago, we showed that thermo-chemically denatured, partially-unfolded forms of Pyrococcus furiosus triosephosphateisomerase (PfuTIM) display cold-denaturation upon cooling, and heat-renaturation upon reheating, in proportion with the extent of ...
Sanjeev Kumar Chandrayan +3 more
doaj +1 more source
FEM1B enhances TRAIL‐induced apoptosis in T lymphocytes and monocytes
FEBS Open Bio, EarlyView.FEM1B facilitates TRAIL‐induced apoptosis through distinct mechanisms in T lymphocytes and monocytes. In T lymphocytes, FEM1B engages with TRAF2, leading to a reduction in TRAF2 expression, which subsequently lessens TRAF2's inhibitory influence on caspase‐8.
Chenbo Yang +5 more
wiley +1 more source
The role of protein denaturation energetics and molecular chaperones in the aggregation and mistargeting of mutants causing primary hyperoxaluria type I. [PDF]
PLoS ONE, 2013 Primary hyperoxaluria type I (PH1) is a conformational disease which result in the loss of alanine:glyoxylate aminotransferase (AGT) function. The study of AGT has important implications for protein folding and trafficking because PH1 mutants may cause ...
Noel Mesa-Torres +6 more
doaj +1 more source
The manufacture of antibodies in vitro [PDF]
, 1942 A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine gamma-globulin and the dye with alkali and then slowly neutralizing the alkali.
Campbell, Dan H., Pauling, Linus
core +2 more sources
FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Cytotoxic Complexes of Sodium Oleate with β-Lactoglobulin [PDF]
, 2011 pre-printA complex of α-lactalbumin and oleic acid has previously been shown to induce apoptosis in cancer cells in a number of in vitro and in vivo trials.
Auty, Mark +7 more
core +1 more source
FEBS Open Bio, EarlyView.In this research, a rat model of asthma was created using OVA, and polydatin served as an intervention. By inhibiting ferroautophagy mediated by NCOA4 and averting ferroptosis, polydatin has been demonstrated to reduce asthma. This work presents new ideas for investigating the mechanism of polydatin's ability to alleviate asthma, in addition to ...
Wei Li +5 more
wiley +1 more source
BioTechniques, 2012 Protein stability can be monitored by many different techniques. However, these protocols are often lengthy, consume large amounts of protein, and require expensive and specialized instruments.
Kyle K. Biggar +2 more
doaj +1 more source
Exploring the denaturation of whey proteins upon application of moderate electric fields : a kinetic and thermodynamic study [PDF]
, 2011 Thermal processing often results in disruption of the native conformation of whey proteins, thus affecting functional properties. The aim of this work was to evaluate the effects of moderate electric fields on denaturation kinetics and thermodynamic ...
Pereira, Ricardo +2 more
core +1 more source