Results 1 to 10 of about 729,118 (264)
Functional protein dynamics in a crystal. [PDF]
Nat Commun, 2023 Abstract Proteins are molecular machines and to understand how they work, we need to understand how they move. New pump-probe time-resolved X-ray diffraction methods open up ways to initiate and observe protein motions with atomistic detail in crystals on biologically relevant timescales.
Klyshko E +6 more
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Targeting Ras-binding domain of ELMO1 by computational nanobody design
Communications Biology, 2023 Structural analysis of the Ras-binding domain (RBD) of ELMO1 alone and in complex with the activator RhoG enables rational engineering and validation of a nanobody targeting ELMO1 RBD.
Chunlai Tam +11 more
doaj +1 more source
Functional Dynamics of Proteins [PDF]
Computational and Mathematical Methods in Medicine, 2012 X-ray crystallography captures protein conformational states that are stable enough to be captured. These observable states are simply part of the functional event in the context of protein dynamics. Life at the molecular level has a vibrant nature at physiological temperature, governed by physics laws. Molecular dynamics (MDs) simulations have been
Lee-Wei Yang +4 more
openaire +2 more sources
Cells, 2022 The dopamine transporter (DAT) is a member of the neurotransmitter:sodium symporter (NSS) family, mediating the sodium-driven reuptake of dopamine from the extracellular space thereby terminating dopaminergic neurotransmission.
Ciara Frances Pugh +3 more
doaj +1 more source
Cells, 2020 Centrioles are microtubule-based cellular structures present in most human cells that build centrosomes and cilia. Proliferating cells have only two centrosomes and this number is stringently maintained through the temporally and spatially controlled ...
Catherine Sullenberger +3 more
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Modern Tools for Rapid Diagnostics of Antimicrobial Resistance
Frontiers in Cellular and Infection Microbiology, 2020 Fast, robust, and affordable antimicrobial susceptibility testing (AST) is required, as roughly 50% of antibiotic treatments are started with wrong antibiotics and without a proper diagnosis of the pathogen. Validated growth-based AST according to EUCAST
Antti Vasala +3 more
doaj +1 more source
A protein quality control pathway at the mitochondrial outer membrane
eLife, 2020 Maintaining the essential functions of mitochondria requires mechanisms to recognize and remove misfolded proteins. However, quality control (QC) pathways for misfolded mitochondrial proteins remain poorly defined.
Meredith B Metzger +4 more
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Reduced efficacy of a Src kinase inhibitor in crowded protein solution
Nature Communications, 2021 The intracellular compartment is a crowded environment. Here, the authors use molecular dynamics (MD) simulations to assess inhibitor binding to c-Src kinase and show how ligand binding pathways differ in crowded and dilute protein solutions ...
Kento Kasahara +10 more
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The dopamine transporter antiports potassium to increase the uptake of dopamine
Nature Communications, 2022 The dopamine transporter, DAT, controls dopamine signaling by facilitating its reuptake using the Na+ gradient as driving force. Here, the authors uncover that an antiport of K+ ions also contributes to setting the rate of DAT-mediated dopamine clearance.
Solveig G. Schmidt +9 more
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Developing a peptide to disrupt cohesin head domain interactions
iScience, 2023 Summary: Cohesin mediates the 3-D structure of chromatin and is involved in maintaining genome stability and function. The cohesin core comprises Smc1 and Smc3, elongated-shaped proteins that dimerize through globular domains at their edges, called head ...
Maria Elias +9 more
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