Improved split fluorescent proteins for endogenous protein labeling. [PDF]
, 2017 Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact.
Feng, Siyu +5 more
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Synthetic biology—putting engineering into biology [PDF]
, 2006 Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Heinemann, Matthias,, Panke, Sven,
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An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics [PDF]
, 2016 The use of protein tagging to facilitate detailed characterization of target proteins has not only revolutionized cell biology, but also enabled biochemical analysis through efficient recovery of the protein complexes wherein the tagged proteins reside ...
De Sutter, Delphine +9 more
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Protein-based materials, toward a new level of structural control [PDF]
, 2001 Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials.
Tirrell, David A., van Hest, Jan C. M.
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Algorithm engineering for optimal alignment of protein structure distance matrices [PDF]
, 2011 Protein structural alignment is an important problem in computational biology. In this paper, we present first successes on provably optimal pairwise alignment of protein inter-residue distance matrices, using the popular Dali scoring function.
A. Andreeva +24 more
core +7 more sources
Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds [PDF]
, 2019 open access articleTissue engineering response may be tailored via controlled, sustained release of active agents from protein-loaded degradable microparticles incorporated directly within three-dimensional (3D) ice-templated collagen scaffolds. However,
Best, S. +6 more
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Comparison of protein quantification and extraction methods suitable for E-coli cultures [PDF]
, 2008 Many different extraction and analysis methods exist to determine the protein fraction of microbial cells. For metabolic engineering purposes it is important to have precise and accurate measurements.
De Mey, Marjan +5 more
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Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates [PDF]
, 2019 Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates.
Ashley, Ben +6 more
core +2 more sources
Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli [PDF]
, 2017 peer-reviewedThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin ...
Cotter, Paul D. +5 more
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Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid [PDF]
, 2008 Bacteriophage P22 serves as a model for the assembly and maturation of other icosahedral double-stranded DNA viruses. P22 coat and scaffolding proteins assemble in vitro into an icosahedral procapsid, which then expands during DNA packaging (maturation).
Abendon +53 more
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