Results 11 to 20 of about 1,281,585 (240)
Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein. [PDF]
, 2014 Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone.
Arpino, JA, Jones, DD, Rizkallah, PJ
core +3 more sources
Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
, 2019 Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi +28 more
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A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution. [PDF]
, 2013 Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures.
Czornyj, Elizabeth +8 more
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The regions of the sequence most exposed to the solvent within the amyloidogenic state of a protein initiate the aggregation process. [PDF]
, 2004 Formation of misfolded aggregates is an essential part of what proteins can do. The process of protein aggregation is central to many human diseases and any aggregating event needs to be prevented within a cell and in protein design.
AMORESANO, ANGELA +6 more
core +1 more source
FEBS Letters, 2013 Improving the stability of proteins is an important goal in many biomedical and industrial applications. A logical approach is to emulate stabilizing molecular interactions found in nature. Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well‐defined geometric conformations, thus making them ...
Dombkowski, Alan A. +2 more
openaire +2 more sources
Machine learning-guided directed evolution for protein engineering [PDF]
, 2019 Machine learning (ML)-guided directed evolution is a new paradigm for biological design that enables optimization of complex functions. ML methods use data to predict how sequence maps to function without requiring a detailed model of the underlying ...
Arnold, Frances H. +2 more
core +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Engineering Nutritious Proteins [PDF]
Biotechnology and Genetic Engineering Reviews, 1997 (1997). Engineering Nutritious Proteins. Biotechnology and Genetic Engineering Reviews: Vol. 14, No. 1, pp. 191-210.
openaire +2 more sources
Peritumoral administration of GPI-anchored TIMP-1 inhibits colon carcinoma growth in Rag-2 gamma chain-deficient mice [PDF]
, 2009 Exogenous application of recombinant TIMP-1 protein modified by addition of a glycosylphosphatidylinositol (GPI) anchor allows efficient insertion of the fusion protein into cell membranes.
Djafarzadeh, Roghieh +6 more
core +1 more source
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source