Results 11 to 20 of about 8,269,032 (242)

Biophysics-based protein language models for protein engineering [PDF]

Nat Methods
Protein language models trained on evolutionary data have emerged as powerful tools for predictive problems involving protein sequence, structure, and function. However, these models overlook decades of research into biophysical factors governing protein
Sam Gelman, Bryce Johnson, Chase R. Freschlin, Arnav Sharma, Sameer D'Costa, John Peters, Anthony Gitter, Philip A. Romero   +7 more
semanticscholar   +2 more sources

A Bivalent COVID-19 Vaccine Based on Alpha and Beta Variants Elicits Potent and Broad Immune Responses in Mice against SARS-CoV-2 Variants

Vaccines, 2022
With the emergence and rapid spread of new pandemic variants, especially variants of concern (VOCs), the development of next-generation vaccines with broad-spectrum neutralizing activities is of great importance.
Rui Wang, Chunyun Sun, Juan Ma, Chulin Yu, Desheng Kong, Meng Chen, Xuejie Liu, Dandan Zhao, Shuman Gao, Shuyuan Kou, Lili Sun, Zeyong Ge, Jun Zhao, Kuokuo Li, Tao Zhang, Yanjing Zhang, Chunxia Luo, Xuefeng Li, Yang Wang, Liangzhi Xie   +19 more
doaj   +1 more source

In vivo pharmacokinetic enhancement of monomeric Fc and monovalent bispecific designs through structural guidance

Communications Biology, 2021
Lu Shan et al. present a structure-guided approach to engineer a monovalent form of the fragment crystallizable (Fc) region of an IgG4 antibody to adapt multiple versions of half-life extension modifications and bispecific targeting.
Lu Shan, Nydia Van Dyk, Nantaporn Haskins, Kimberly M. Cook, Kim L. Rosenthal, Ronit Mazor, Sonia Dragulin-Otto, Yu Jiang, Herren Wu, William F. Dall’Acqua, Martin J. Borrok, Melissa M. Damschroder, Vaheh Oganesyan   +12 more
doaj   +1 more source

FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner

Cell Communication and Signaling, 2020
Fibroblast growth factors (FGFs) via their receptors (FGFRs) transduce signals from the extracellular space to the cell interior, modulating pivotal cellular processes such as cell proliferation, motility, metabolism and death. FGF superfamily includes a
Martyna Sochacka, Lukasz Opalinski, Jakub Szymczyk, Marta B. Zimoch, Aleksandra Czyrek, Daniel Krowarsch, Jacek Otlewski, Malgorzata Zakrzewska   +7 more
doaj   +1 more source

N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope

Cell Communication and Signaling, 2023
Fibroblast growth factor receptor 1 (FGFR1) is a heavily N-glycosylated cell surface receptor tyrosine kinase that transmits signals across the plasma membrane, in response to fibroblast growth factors (FGFs).
Paulina Gregorczyk, Natalia Porębska, Dominika Żukowska, Aleksandra Chorążewska, Aleksandra Gędaj, Agata Malinowska, Jacek Otlewski, Małgorzata Zakrzewska, Łukasz Opaliński   +8 more
doaj   +1 more source

Low-N protein engineering with data-efficient deep learning

Nature Methods, 2020
Protein engineering has enormous academic and industrial potential. However, it is limited by the lack of experimental assays that are consistent with the design goal and sufficiently high throughput to find rare, enhanced variants.
Surojit Biswas, Grigory Khimulya, E. C. Alley, K. Esvelt, G. Church   +4 more
semanticscholar   +1 more source

Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

Nature Communications, 2021
Random mutagenesis is a technique used to generate diversity and engineer biological systems. In vivo random mutagenesis generates diversity directly in a host organism, enabling applications such as lineage tracing, continuous evolution, and protein ...
Aaron Cravens, Osman K Jamil, Deze Kong, Jonathan T. Sockolosky, C. Smolke   +4 more
semanticscholar   +1 more source

Protein Engineering Strategies for Improved Pharmacokinetics

Advanced Functional Materials, 2021
Protein therapeutics have gained momentum in recent years and become a pillar in treating many diseases and the only choice in several ailments. Protein therapeutics are highly specific, tunable, and less toxic than conventional small drug molecules ...
Aurélie Rondon, Sohaib Mahri, Francisco Morales-Yánez, M. Dumoulin, R. Vanbever   +4 more
semanticscholar   +1 more source

Identification of a novel peptide ligand for the cancer-specific receptor mutation EGFRvIII using high-throughput sequencing of phage-selected peptides

Scientific Reports, 2022
We report here the selection and characterization of a novel peptide ligand using phage display targeted against the cancer-specific epidermal growth factor tyrosine kinase receptor mutation variant III (EGFRvIII).
Sourour Mansour, Indranil Adhya, Coralie Lebleu, Rama Dumpati, Ahmed Rehan, Santu Chall, Jingqi Dai, Gauthier Errasti, Thomas Delacroix, Raj Chakrabarti   +9 more
doaj   +1 more source

A Polysaccharide-RBD-Fc-Conjugated COVID-19 Vaccine, SCTV01A, Showed High Immunogenicity and Low Toxicity in Animal Models

Vaccines, 2023
We previously developed a polysaccharide-–RBD-conjugated nanoparticle vaccine which induced protective efficacy against SARS-CoV-2 in a mouse model. Here, we newly developed a vaccine, SCTV01A, by chemically conjugating recombinant SARS-CoV-2 RBD-Fc and ...
Chunyun Sun, Desheng Kong, Erhong Guo, Jun Zhao, Jilei Jia, Rui Wang, Juan Ma, Meng Chen, Jianbo Lu, Chulin Yu, Kuokuo Li, Liangzhi Xie   +11 more
doaj   +1 more source