Results 261 to 270 of about 8,260,919 (293)

Mapping protein–protein interactions by mass spectrometry

Current Opinion in Biotechnology, 2006
Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction ...
Julian, Vasilescu, Daniel, Figeys
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Protein‐Protein Interaction Mapping by 2C‐BioID

Current Protocols in Cell Biology, 2019
AbstractProtein‐protein interactions (PPIs) add an essential layer of complexity to the information encoded by the genome. Modulation of such interactions is a key feature of most, if not all, cellular activities and allows cells to respond rapidly to both internal and external signals and stimuli. In this respect, the development of the BioID assay to
Alexandre, Chojnowski, Hendrikje, Werner, Matthew, Cook, Radoslaw M, Sobota, Brian, Burke, Colin L, Stewart   +5 more
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Deconvolution of Targeted Protein–Protein Interaction Maps

Journal of Proteome Research, 2012
Current proteomic techniques allow researchers to analyze chosen biological pathways or an ensemble of related protein complexes at a global level via the measure of physical protein-protein interactions by affinity purification mass spectrometry (AP-MS). Such experiments yield information-rich but complex interaction maps whose unbiased interpretation
Alexey, Stukalov, Giulio, Superti-Furga, Jacques, Colinge   +2 more
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Progress and Potential of Drosophila Protein Interaction Maps

Pharmacogenomics, 2000
Protein-protein interactions mediate many important cellular processes and are central to the mechanisms by which most proteins function. Charting the interactions among the proteins involved in a process has been an essential step in characterising the function of proteins and pathways.
Clement Stanyon, Russell Finley
exaly   +3 more sources

Deciphering complex protein interaction kinetics using Interaction Map

Biochemical and Biophysical Research Communications, 2012
Cellular receptor systems are expected to present complex ligand interaction patterns that cannot be evaluated assuming a simple one ligand:one receptor interaction model. We have previously evaluated heterogeneous interactions using an alternative method to regression analysis, called Interaction Map (IM).
Altschuh, D, Bjorkelund, H, Strandgard, J, Choulier, Laurence, Malmqvist, M, Andersson, K   +5 more
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Mapping protein–protein interactions with combinatorial biology methods

Current Opinion in Biotechnology, 2001
Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA.
J, Pelletier, S, Sidhu
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Proteolytic DNA for Mapping Protein−DNA Interactions

Biochemistry, 2002
We describe a technique to determine sites on proteins involved in protein-DNA interactions. DNA was synthesized via polymerase chain reaction (PCR) to produce four polynucleotide products with phosphorothioate nucleotides at the A, T, G, or C residues.
Brian D, Schmidt, Claude F, Meares
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Transcriptome-Wide Mapping of Protein–RNA Interactions

2020
RNA and RNA-binding proteins (RBPs) control multiple biological processes. The spatial and temporal arrangement of RNAs and RBPs underlies the delicate regulation of these processes. The strategy called CLIP-seq (cross-linking and immunoprecipitation) has been developed to capture endogenous protein-RNA interactions with UV cross-linking followed by ...
Xianju, Bi, Xiaohua, Shen
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Construction and Prediction of Protein-Protein Interaction Maps

2002
With the completion of full genome sequence for several model organisms, new approaches are emerging to comprehensively characterize the function of gene products. In these so-called functional proteomics approaches, large-scale assays on the complete set of proteins of a given organism — the proteome — enable the study of the function of proteins in ...
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A Protein Interaction Map of Drosophila melanogaster

Science, 2003
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map
L, Giot, J S, Bader, C, Brouwer, A, Chaudhuri, B, Kuang, Y, Li, Y L, Hao, C E, Ooi, B, Godwin, E, Vitols, G, Vijayadamodar, P, Pochart, H, Machineni, M, Welsh, Y, Kong, B, Zerhusen, R, Malcolm, Z, Varrone, A, Collis, M, Minto, S, Burgess, L, McDaniel, E, Stimpson, F, Spriggs, J, Williams, K, Neurath, N, Ioime, M, Agee, E, Voss, K, Furtak, R, Renzulli, N, Aanensen, S, Carrolla, E, Bickelhaupt, Y, Lazovatsky, A, DaSilva, J, Zhong, C A, Stanyon, R L, Finley, K P, White, M, Braverman, T, Jarvie, S, Gold, M, Leach, J, Knight, R A, Shimkets, M P, McKenna, J, Chant, J M, Rothberg   +48 more
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