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Deconvolution of Targeted Protein–Protein Interaction Maps
Journal of Proteome Research, 2012Current proteomic techniques allow researchers to analyze chosen biological pathways or an ensemble of related protein complexes at a global level via the measure of physical protein-protein interactions by affinity purification mass spectrometry (AP-MS). Such experiments yield information-rich but complex interaction maps whose unbiased interpretation
Alexey Stukalov +2 more
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Mapping Protein–Protein Interactions by Quantitative Proteomics
2010Proteins exert their function inside a cell generally in multiprotein complexes. These complexes are highly dynamic structures changing their composition over time and cell state. The same protein may thereby fulfill different functions depending on its binding partners.
Dengjel, Jörn +2 more
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Mapping of RNA–protein interactions
Analytica Chimica Acta, 2009RNA-protein interactions are important biological events that perform multiple functions in all living organisms. The wide range of RNA interactions demands diverse conformations to provide contacts for the selective recognition of proteins. Various analytical procedures are presently available for quantitative analyses of RNA-protein complexes, but ...
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Mapping protein–protein interactions by mass spectrometry
Current Opinion in Biotechnology, 2006Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction ...
Julian, Vasilescu, Daniel, Figeys
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Protein‐Protein Interaction Mapping by 2C‐BioID
Current Protocols in Cell Biology, 2019AbstractProtein‐protein interactions (PPIs) add an essential layer of complexity to the information encoded by the genome. Modulation of such interactions is a key feature of most, if not all, cellular activities and allows cells to respond rapidly to both internal and external signals and stimuli. In this respect, the development of the BioID assay to
Alexandre, Chojnowski +5 more
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Novel approaches to map protein interactions
Current Opinion in Biotechnology, 2003Although we now have the sequence of the human genome at hand, we face the challenge of assigning function to the identified genes. Genes usually ascribe their function through proteins, and the role of proteins is to interact with other molecules. Therefore, if we could map the interactions of proteins we would be able to understand protein function ...
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Progress and potential of Drosophila protein interaction maps
Pharmacogenomics, 2000Protein-protein interactions mediate many important cellular processes and are central to the mechanisms by which most proteins function. Charting the interactions among the proteins involved in a process has been an essential step in characterising the function of proteins and pathways.
Russell L Finley
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The protein–protein interaction map of Helicobacter pylori
Nature, 2001With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function. As elementary constituents of cellular protein complexes and pathways, protein-protein interactions are key ...
J C, Rain +12 more
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Deciphering complex protein interaction kinetics using Interaction Map
Biochemical and Biophysical Research Communications, 2012Cellular receptor systems are expected to present complex ligand interaction patterns that cannot be evaluated assuming a simple one ligand:one receptor interaction model. We have previously evaluated heterogeneous interactions using an alternative method to regression analysis, called Interaction Map (IM).
Danièle, Altschuh +5 more
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Proteolytic DNA for Mapping Protein−DNA Interactions
Biochemistry, 2002We describe a technique to determine sites on proteins involved in protein-DNA interactions. DNA was synthesized via polymerase chain reaction (PCR) to produce four polynucleotide products with phosphorothioate nucleotides at the A, T, G, or C residues.
Brian D, Schmidt, Claude F, Meares
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