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Isolation of UVR8 Protein Complexes
2021The fundamental mechanism of light regulated plant development involves photoreceptors and their interacting proteins which act as light signaling intermediate factors. In Arabidopsis thaliana, UV RESISTANCE LOCUS 8 (UVR8) is responsible for the perception and the initiation of UV-B light signal.
Yan, Liu, Xi, Huang
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2006
Proteome analysis is becoming a powerful tool of discovery-driven research, with investigations ranging from whole organisms to specific subcellular compartments. Especially for the latter, efficient and robust methods for protein purification are the prerequisite for obtaining meaningful proteomic data.
Tomasz T, Calikowski, Iris, Meier
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Proteome analysis is becoming a powerful tool of discovery-driven research, with investigations ranging from whole organisms to specific subcellular compartments. Especially for the latter, efficient and robust methods for protein purification are the prerequisite for obtaining meaningful proteomic data.
Tomasz T, Calikowski, Iris, Meier
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Protein synthesis by isolated chloroplasts
Photosynthesis Research, 1988Isolated chloroplasts show substantial rates of protein synthesis when illuminated. This 'in organello' protein synthesis system has been advantageously utilised to elucidate the coding capacity of chloroplast and the regulation of chloroplast genes.
A, Gnanam, C C, Subbaiah, R M, Mannan
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Isolation of cadmium-binding proteins
Experientia, 1971Un procede pour l'isolation des proteines combinees au Cd radioactif (109Cd) a ete mis au point. La methode ne detruit pas les proteines. D'autres proteines ayant des affinites pour les metaux lourds pourront ainsi etre isolees.
Z A, Shaikh, O J, Lucis
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Isolation of Bacteria Envelope Proteins
2012Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques. We found that conventional extraction methods such as osmotic shock cause extracts to be heavily contaminated with soluble cytoplasmic proteins. These cytoplasmic protein contaminants constitute the major signal in proteomic analysis and can
Shu, Quan +3 more
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Protein Import Into Isolated Mitochondria
2007Import of proteins into mitochondria is a pivotal process in the biogenesis of mitochondria. Only about 1% of the 1000-2000 different proteins constituting the mitochondrion are encoded in the mitochondrial deoxyribonucleic acid (DNA). All others are specified by nuclear genes. They are translated in the cytosol and released from ribosomes as precursor
Dejana, Mokranjac, Walter, Neupert
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Chemistry of Natural Compounds, 1983
The seeds of the cotton plant are an important source of food protein. The presence of gossypol in the seeds limits the use of protein isolates as additives to food products. The use of salt and alkaline solutions for extracting the proteins leads to the formation of isolates with relatively high gossypol constants. Extraction in an acid medium permits
T. S. Yunusov, M. T. Turakhozhaev
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The seeds of the cotton plant are an important source of food protein. The presence of gossypol in the seeds limits the use of protein isolates as additives to food products. The use of salt and alkaline solutions for extracting the proteins leads to the formation of isolates with relatively high gossypol constants. Extraction in an acid medium permits
T. S. Yunusov, M. T. Turakhozhaev
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Isolation of Proteins from Polyacrylamide Gels
2012Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
Koristka, S. +3 more
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Affinity chromatography for protein isolation
Current Opinion in Biotechnology, 1991Thousands of reports concerning protein purification have appeared in the past year, and over 150 of these involved, at least in part, the affinity chromatography process. Immobilized membrane affinity chromatography, temperature-programmed elution, and centrifugal affinity chromatography are among the most significant new techniques amid the myriads ...
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Isolation of Recombinant DNA Elongation Proteins
2009This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) delta, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d) replication factor C (RF-C).
van Loon, B, Ferrari, E, Hübscher, U
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