Results 211 to 220 of about 499,653 (267)
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Isolation of Microtubules and Microtubule Proteins
Current Protocols in Cell Biology, 2008AbstractThis unit describes various protocols for the isolation and purification of the main constituents of microtubules, chiefly α‐ and β‐tubulin, and the most significant microtubule associated proteins (MAPs), specifically MAP1A, MAP1B, MAP2, and tau.
Ávila, Jesús +3 more
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2006
Proteome analysis is becoming a powerful tool of discovery-driven research, with investigations ranging from whole organisms to specific subcellular compartments. Especially for the latter, efficient and robust methods for protein purification are the prerequisite for obtaining meaningful proteomic data.
Tomasz T, Calikowski, Iris, Meier
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Proteome analysis is becoming a powerful tool of discovery-driven research, with investigations ranging from whole organisms to specific subcellular compartments. Especially for the latter, efficient and robust methods for protein purification are the prerequisite for obtaining meaningful proteomic data.
Tomasz T, Calikowski, Iris, Meier
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Protein synthesis by isolated chloroplasts
Photosynthesis Research, 1988Isolated chloroplasts show substantial rates of protein synthesis when illuminated. This 'in organello' protein synthesis system has been advantageously utilised to elucidate the coding capacity of chloroplast and the regulation of chloroplast genes.
A, Gnanam, C C, Subbaiah, R M, Mannan
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Isolation of Proteins and Protein Complexes by Immunoprecipitation
2008Immunoprecipitation (IP) uses the specificity of antibodies to isolate target proteins (antigens) out of complex sample mixtures. Three different approaches for performing IP will be discussed; traditional (classical) method, oriented affinity method and direct affinity method.
Barbara, Kaboord, Maria, Perr
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Isolation of cadmium-binding proteins
Experientia, 1971Un procede pour l'isolation des proteines combinees au Cd radioactif (109Cd) a ete mis au point. La methode ne detruit pas les proteines. D'autres proteines ayant des affinites pour les metaux lourds pourront ainsi etre isolees.
Z A, Shaikh, O J, Lucis
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Photoionization of a protein isolated in vacuo
Physical Chemistry Chemical Physics, 2011Gas phase VUV single-photon photoionization spectroscopy of electrospray-produced multiply protonated cytochrome c protein (12 kDa) has been performed by means of coupling a linear quadrupole ion trap with a synchrotron beamline. The thresholds for the ionization of the 8+ and 11+ charge state precursors to radical 9+ and 12+ species were measured to ...
Milosavljević, Aleksandar R. +8 more
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Affinity chromatography for protein isolation
Current Opinion in Biotechnology, 1991Thousands of reports concerning protein purification have appeared in the past year, and over 150 of these involved, at least in part, the affinity chromatography process. Immobilized membrane affinity chromatography, temperature-programmed elution, and centrifugal affinity chromatography are among the most significant new techniques amid the myriads ...
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Isolation of Bacteria Envelope Proteins
2012Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques. We found that conventional extraction methods such as osmotic shock cause extracts to be heavily contaminated with soluble cytoplasmic proteins. These cytoplasmic protein contaminants constitute the major signal in proteomic analysis and can
Shu, Quan +3 more
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Isolation of Proteins for Microsequence Analysis
Current Protocols in Molecular Biology, 1992AbstractThis unit presents a method for determining the amino‐terminal sequence of polypeptides or proteins. It is particularly appropriate for large fragments of insoluble or hydrophobic proteins or proteins that cannot be purified to >90% molar purity without electrophoresis.
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Protein Import Into Isolated Mitochondria
2007Import of proteins into mitochondria is a pivotal process in the biogenesis of mitochondria. Only about 1% of the 1000-2000 different proteins constituting the mitochondrion are encoded in the mitochondrial deoxyribonucleic acid (DNA). All others are specified by nuclear genes. They are translated in the cytosol and released from ribosomes as precursor
Dejana, Mokranjac, Walter, Neupert
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