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Isolation of Recombinant DNA Elongation Proteins
2009This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) delta, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d) replication factor C (RF-C).
van Loon, B, Ferrari, E, Hübscher, U
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International Journal of Biological Macromolecules
The influence of food components on the physicochemical properties and biological responses of nanoparticles is pivotal. Since soy protein isolate (SPI) and whey protein isolate (WPI) are commonly used as plant and animal proteins in food, it is imperative to gain comprehensive knowledge of the interaction between SPI/WPI and nanoparticles. Accordingly,
Jie, Yang +6 more
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The influence of food components on the physicochemical properties and biological responses of nanoparticles is pivotal. Since soy protein isolate (SPI) and whey protein isolate (WPI) are commonly used as plant and animal proteins in food, it is imperative to gain comprehensive knowledge of the interaction between SPI/WPI and nanoparticles. Accordingly,
Jie, Yang +6 more
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Immunological Technique for Protein Isolation
Science, 1960Carefully coagulated antibody or antigen-antibody complexes may be used as specific adsorbents for antigen, and the antigen may be released subsequently by increasing the acidity. No cross adsorption appears to take place. The procedure may prove useful for the isolation of tissue-specific proteins (including those in disease states), toxins, or ...
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Isolation of Proteins from Polyacrylamide Gels
2012Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
Koristka, S. +3 more
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The phenolic profile of walnut meal protein isolate and interaction of phenolics with walnut protein
Food Research International, 2023Chi Chen, Xudong Zhang, Xiangjun Sun
exaly
Overexpression, Isolation, and Crystallization of Proteins
2003J V, Skelly, C B, Madden
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