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Protein synthesis with isolated mitochondrial polysomes
Biochemical and Biophysical Research Communications, 1972A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template.
N G, Avadhani, D E, Buetow
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Immunological Technique for Protein Isolation
Science, 1960Carefully coagulated antibody or antigen-antibody complexes may be used as specific adsorbents for antigen, and the antigen may be released subsequently by increasing the acidity. No cross adsorption appears to take place. The procedure may prove useful for the isolation of tissue-specific proteins (including those in disease states), toxins, or ...
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Multipurpose peptide tags for protein isolation
Journal of Chromatography A, 2008A multifunctional peptide tag (HYDHYD) consisting of histidine, tyrosine and aspartate residues was fused to the N-terminal ends of green fluorescent protein (GFP), lactate dehydrogenase (LDH) and human hemoglobin (Hb), proteins which were subjected to ion-exchange chromatography (IEC), aqueous two-phase system partition, immobilized metal-ion affinity
Kristian, Becker +2 more
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Isolation of recombinant proteins from milk
Journal of Cellular Biochemistry, 1992AbstractMilk is a complex bio‐colloid which presents some unique problems for the protein isolation chemist, but the majority of the processing criteria for purifying recombinant proteins are the same as with any complex biological mixture. The casein micelles and fat globules behave as separate phases; they prevent filtration of the milk and interfere
T D, Wilkins, W, Velander
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Protein variability among Mycoplasma hyopneumoniae isolates
Veterinary Microbiology, 2007Sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to study the protein variability of Mycoplasma hyopneumoniae isolates. Fifty-six M. hyopneumoniae isolates from 6 different countries and 37 different herds were used. From eight herds, more than one isolate was available.
D, Calus +7 more
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Isolation of Heat Shock Protein Complexes
2011Heat shock proteins (Hsp) are molecular chaperones with the capability to interact with a wide range of other proteins and are thus often found coupled with other heat shock and non-heat shock proteins. This can be an advantage to study specific interactions between a chaperone and other proteins and to generate an antitumoral immune response.
Fernando D, Cuello-Carrión +4 more
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Isolating DNA, RNA, Polysomes, and Protein
2004Publisher Summary The chapter outlines methods for preparing RNA, DNA, polysomes, and proteins from the embryos or sperm of Strongylocentrotus purpuratus , which are widely applicable to the embryos of many other marine species. The methods described in the chapter facilitate analysis of gene expression that focuses on the synthesis, presence, and ...
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Pea protein isolate production
2020ABSTRACT PEA PROTEIN ISOLATE PRODUCTION GÜRGEN, Emre M. Sc, Department of Food Engineering Supervisor: Prof. Dr. Suat UNGAN August 2005, 69 pages Pea seeds were tempered at moisture contents of 12.0±0.1, 13.0±0.1, 14.0+.0.1 and 15.0±0.3%. The seeds with different moisture contents were then milled and fractioned according to the particle size of 53 ...
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