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Protein synthesis with isolated mitochondrial polysomes
Biochemical and Biophysical Research Communications, 1972A technique has been described for the isolation of intact mitochondrial polysomes which are active in protein synthesis. Mitochondrial ribosomes, derived from the polysomes, are of 71S particles and have 32S and 50S subunits. These subunits can initiate protein synthesis using mitochondrial RNA as a template.
N G, Avadhani, D E, Buetow
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Journal of Agricultural and Food Chemistry, 2019
At present, the structure-activity relationships of soy protein isolate are still not well understood. In this paper, the relationship between molecular flexibility and emulsifying properties of soy protein isolate and soy protein isolate-glucose ...
Rui Li +6 more
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At present, the structure-activity relationships of soy protein isolate are still not well understood. In this paper, the relationship between molecular flexibility and emulsifying properties of soy protein isolate and soy protein isolate-glucose ...
Rui Li +6 more
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Food Chemistry, 2019
In this study, the effects of the addition of various polysaccharides (konjac gum, gellan gum, and curdlan gum) on the rheological and textural properties of calcium sulfate-induced soy protein isolate gels were investigated.
Haibo Zhao, Jie Chen, Y. Hemar, Bo Cui
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In this study, the effects of the addition of various polysaccharides (konjac gum, gellan gum, and curdlan gum) on the rheological and textural properties of calcium sulfate-induced soy protein isolate gels were investigated.
Haibo Zhao, Jie Chen, Y. Hemar, Bo Cui
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Immunological Technique for Protein Isolation
Science, 1960Carefully coagulated antibody or antigen-antibody complexes may be used as specific adsorbents for antigen, and the antigen may be released subsequently by increasing the acidity. No cross adsorption appears to take place. The procedure may prove useful for the isolation of tissue-specific proteins (including those in disease states), toxins, or ...
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Multipurpose peptide tags for protein isolation
Journal of Chromatography A, 2008A multifunctional peptide tag (HYDHYD) consisting of histidine, tyrosine and aspartate residues was fused to the N-terminal ends of green fluorescent protein (GFP), lactate dehydrogenase (LDH) and human hemoglobin (Hb), proteins which were subjected to ion-exchange chromatography (IEC), aqueous two-phase system partition, immobilized metal-ion affinity
Kristian, Becker +2 more
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Quality characterization of gluten free noodles enriched with chickpea protein isolate
, 2020Gluten free noodles were developed from rice flour with enhanced protein content through incorporation of protein isolate (2, 4, 6, 8, and 10%) from two germinated chickpea cultivars (GNG 1581 and GNG 469).
S. Sofi +4 more
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Isolation of recombinant proteins from milk
Journal of Cellular Biochemistry, 1992AbstractMilk is a complex bio‐colloid which presents some unique problems for the protein isolation chemist, but the majority of the processing criteria for purifying recombinant proteins are the same as with any complex biological mixture. The casein micelles and fat globules behave as separate phases; they prevent filtration of the milk and interfere
T D, Wilkins, W, Velander
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Food Chemistry, 2019
Covalent modification of flaxseed protein isolate by phenolic compounds including flaxseed polyphenols, ferulic acid, and hydroxytyrosol was studied under alkaline condition and in the presence of oxygen.
L. Pham, Bo Wang, B. Zisu, B. Adhikari
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Covalent modification of flaxseed protein isolate by phenolic compounds including flaxseed polyphenols, ferulic acid, and hydroxytyrosol was studied under alkaline condition and in the presence of oxygen.
L. Pham, Bo Wang, B. Zisu, B. Adhikari
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