Results 51 to 60 of about 30,399 (190)

De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist

iScience, 2021
Summary: Lasso-grafting (LG) technology is a method for generating de novo biologics (neobiologics) by genetically implanting macrocyclic peptide pharmacophores, which are selected in vitro against a protein of interest, into loops of arbitrary protein ...
Yamato Komatsu, Naohiro Terasaka, Katsuya Sakai, Emiko Mihara, Risa Wakabayashi, Kunio Matsumoto, Donald Hilvert, Junichi Takagi, Hiroaki Suga   +8 more
doaj   +1 more source

Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains

Cytoskeleton, EarlyView.
ABSTRACT Plant cells create a plasma membrane‐associated network of microtubules that are nucleated by γ‐tubulin ring complexes primarily through microtubule‐dependent microtubule nucleation (MDMN). This dynamic array organizes into specific patterns in response to developmental and environmental cues to influence primary cell wall construction.
Naveen K. Chana, Timothy Cioffi, Sidney L. Shaw   +2 more
wiley   +1 more source

DockGame: Cooperative Games for Multimeric Rigid Protein Docking

CoRR, 2023
Protein interactions and assembly formation are fundamental to most biological processes. Predicting the assembly structure from constituent proteins -- referred to as the protein docking task -- is thus a crucial step in protein design applications. Most traditional and deep learning methods for docking have focused mainly on binary docking, following
Vignesh Ram Somnath, Pier Giuseppe Sessa, María Rodríguez Martínez, Andreas Krause 0001   +3 more
openaire   +2 more sources

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

The oncogenic fusion protein EML4-NTRK3 requires three salt bridges for stability and biological activity

Heliyon
Aim of study: Chromosomal translocations involving neurotrophic receptor tyrosine kinases (NTRKs) have been identified in 20 % of soft tissue sarcomas. This work focuses on the EML4-NTRK3 translocation identified in cases of Infantile Fibrosarcoma, which
Zian Jiang, April N. Meyer, Wei Yang, Daniel J. Donoghue   +3 more
doaj   +1 more source

Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein-Protein Interactions in Bacteria

mBio, 2014
Protein-protein interactions are important for virtually every biological process, and a number of elegant approaches have been designed to detect and evaluate such interactions.
Boyu Cui, Yao Wang, Yunhong Song, Tietao Wang, Changfu Li, Yahong Wei, Zhao-Qing Luo, Xihui Shen   +7 more
doaj   +1 more source

Mosaic activation of the zebrafish hsp70l heat shock promoter: Implications for interpreting transgenes

Developmental Dynamics, EarlyView.
Abstract Background The promoters and enhancers of heat shock genes, such as the 1.5‐kb promoter of the zebrafish hsp70l gene, are valuable tools for temporal activation of transgenes. It has been widely purported that heat shock treatments result in ubiquitous expression of hsp70l‐driven transgenes.
Jong‐Su Park, Xiangyun Wei
wiley   +1 more source

Nanobodies as next‐generation targeting platforms: From discovery technologies to translational biomedicine

VIEW, EarlyView.
Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley   +1 more source

The DynDom3D Webserver for the Analysis of Domain Movements in Multimeric Proteins

Journal of Computational Biology, 2016
DynDom3D is a program for the analysis of domain movements in multimeric proteins. Its inputs are two structure files that indicate a possible domain movement, but the onus has been on the user to process the files so that there is the necessary one-to-one equivalence between atoms in the two atom lists.
Christopher Girdlestone, Steven Hayward
openaire   +4 more sources

Behind the scenes: how the EMILIN/Multimerin family shapes the cancer landscape

The FEBS Journal, EarlyView.
The EMILIN/Multimerin family members regulate key hallmarks of cancer—including apoptosis, angiogenesis, metastasis, and tumor microenvironment remodeling. As indicated, their function in immune evasion, drug resistance, and metabolic reprogramming remains largely unexplored.
Evelina Poletto, Naike Casagrande, Emanuele Di Siena, Greta Carobolante, Lucrezia Camicia, Giorgia Schinello, Samanta Muzzin, Enrica Timis, Paola Spessotto, Maurizio Mongiat   +9 more
wiley   +1 more source