Results 61 to 70 of about 30,399 (190)

Multimeric Ionotropic Purinoceptor Detection by Protein Cross-Linking [PDF]

, 2019
P2X receptor subunits (P2X1 to P2X7) assemble to form trimeric homomers or heteromers. Here, we describe the use of protein cross-linking to study the composition of P2X receptor complexes. This simple protocol is useful for determining the stoichiometry of P2X heteromeric receptors as well as for assessing the effect of point mutation, truncation, or ...
Vincent, Compan, François, Rassendren
openaire   +2 more sources

Characterization of Hantavirus N Protein Intracellular Dynamics and Localization

Viruses, 2022
Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation,
Robert-William Welke, Hannah Sabeth Sperber, Ronny Bergmann, Amit Koikkarah, Laura Menke, Christian Sieben, Detlev H. Krüger, Salvatore Chiantia, Andreas Herrmann, Roland Schwarzer   +9 more
doaj   +1 more source

An update on the landscape of collagen bioactive fragments

The FEBS Journal, EarlyView.
The remodeling of the extracellular matrix releases collagen bioactive fragments, which exert molecular functions and regulate numerous biological processes via several signaling pathways. Here, we summarize the latest findings describing the roles of major bioactive fragments from collagens I, IV, VI, and XVIII in various physiological and ...
Sylvie Ricard‐Blum, Julie Fradette
wiley   +1 more source

Towards an integrated molecular understanding of plant hormones

Journal of Integrative Plant Biology, EarlyView.
This review offers a comprehensive overview of the nine plant hormones, delving into their biosynthesis, transport, signaling and crosstalk mechanisms. Because the complexity of plant hormonal control goes beyond these core elements, additional specific features are discussed. Lastly, this review highlights how fundamental insights drive hormonal‐based
Louise Vilain, Marie‐Laure Fauconnier, Manon Genva   +2 more
wiley   +1 more source

Chain‐Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis

Molecular Microbiology, EarlyView.
Here, we demonstrate that polysaccharide chain length regulation plays a distinct role in enabling formation of a cyclic molecule important for the bacterial cell surface. However, this function is necessary, but not sufficient for the cyclic molecule's biosynthesis.
Joseph F. Carr, Yohannes H. Rezenom, Jennifer S. Rudolf, Daniel J. Warzecha, Angela M. Mitchell   +4 more
wiley   +1 more source

Brighter Reporter Genes from Multimerized Fluorescent Proteins

BioTechniques, 2005
If low signal-to-noise ratios in GFP assays have you singing a sad tune, Genové et al. (p. 814) can offer a solution. Recognizing that GFP fluorescence can be almost undetectable when reporters are used downstream of relatively weak promoters or during detection in problematic tissues such as the CNS, the authors investigated whether tandem repeats of ...
Guillem Genové, Benjamin S. Glick, Alison L. Barth   +2 more
openaire   +3 more sources

The HIVToolbox 2 web system integrates sequence, structure, function and mutation analysis. [PDF]

PLoS ONE, 2014
There is enormous interest in studying HIV pathogenesis for improving the treatment of patients with HIV infection. HIV infection has become one of the best-studied systems for understanding how a virus can hijack a cell. To help facilitate discovery, we
David P Sargeant, Sandeep Deverasetty, Christy L Strong, Izua J Alaniz, Alexandria Bartlett, Nicholas R Brandon, Steven B Brooks, Frederick A Brown, Flaviona Bufi, Monika Chakarova, Roxanne P David, Karlyn M Dobritch, Horacio P Guerra, Michael W Hedden, Rma Kumra, Kelvy S Levitt, Kiran R Mathew, Ray Matti, Dorothea Q Maza, Sabyasachy Mistry, Nemanja Novakovic, Austin Pomerantz, Josue Portillo, Timothy F Rafalski, Viraj R Rathnayake, Noura Rezapour, Sarah Songao, Sean L Tuggle, Sandy Yousif, David I Dorsky, Martin R Schiller   +30 more
doaj   +1 more source

Technologies for engineering repetitive DNA

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract Repetitive DNA, a fundamental architectural element of genomes, is widespread across organisms and comprises about 54% of the human genome. With advances in long‐read sequencing and bioinformatics approaches, highly repetitive sequences can now be characterized in depth.
Shuting Ma, Yali Cui, Yi Wu
wiley   +1 more source

Pretargeted Imaging with Gallium-68—Improving the Binding Capability by Increasing the Number of Tetrazine Motifs

Pharmaceuticals, 2018
The inverse electron-demand Diels-Alder reaction between 1,2,4,5-tetrazine (Tz) and trans-cyclooct-2-ene (TCO) has gained increasing attraction among extensive studies on click chemistry due to its exceptionally fast reaction kinetics and high ...
Dominik Summer, Sonja Mayr, Milos Petrik, Christine Rangger, Katia Schoeler, Lisa Vieider, Barbara Matuszczak, Clemens Decristoforo   +7 more
doaj   +1 more source

Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions

Molecules, 2020
In comparison with the pervasive use of protein dimers and multimers in all domains of life, functional RNA oligomers have so far rarely been observed in nature.
Charles Bou-Nader, Jinwei Zhang
doaj   +1 more source