Results 101 to 110 of about 4,194,516 (299)

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function

FEBS Open Bio, EarlyView.
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley, Luke W. Molesworth, Nethma H. Waduge, Fahimeh Eskandari, Peter R. Boag, Gregory M. Davis   +5 more
wiley   +1 more source

Renal protein phosphatase 2A activity and spontaneous hypertension in rats

, 2000
The impaired renal paracrine function of dopamine in spontaneously hypertensive rats (SHR) is caused by hyperphosphorylation and desensitization of the renal D1 dopamine receptor. Protein phosphatase 2A (PP(2A)) is critical in the regulation of G-protein
Hopfer, Ulrich, Asico, Laureano D., Eisner, Gilbert M., Yu, Peiying, Felder, Robin A., Jose, Pedro A.   +5 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

The activity of the STriatal-Enriched protein tyrosine Phosphatase (STEP) in neuronal cells is modulated by adenosine A(2A) receptor

, 2020
We recently demonstrated that a tonic activation of adenosine A(2A) receptors (A(2A) Rs) is required for cocaine-induced synaptic depression and increase in the activity of STriatal-Enriched protein tyrosine Phosphatase (STEP).
Domenici, M.R., Paul J. Lombroso, Valentina Chiodi, Patrizia Popoli, Visentin, S., Rita Pepponi, Michael Bader, Chiodi, V., Pepponi, R., Popoli, P., Cinzia Mallozzi, Lombroso, P.J., Maria Rosaria Domenici, Mallozzi, C., Bader, M., Sergio Visentin   +15 more
core   +1 more source

Overexpression of SET is a recurrent event associated with poor outcome and contributes to protein phosphatase 2A inhibition in acute myeloid leukemia

Haematologica, 2012
Background Protein phosphatase 2A is a novel potential therapeutic target in several types of chronic and acute leukemia, and its inhibition is a common event in acute myeloid leukemia.
Ion Cristóbal, Laura Garcia-Orti, Cristina Cirauqui, Xabier Cortes-Lavaud, María A. García-Sánchez, María J. Calasanz, María D. Odero   +6 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Discovery of Protein Phosphatase 2A Substrates

The FASEB Journal, 2018
The protein ser/thr phosphatases (PSPs) dephosphorylate 1000s of key biological targets, making them attractive therapeutic targets. The serine/threonine protein phosphatases 1 and 2B (PP1; PP2B or calcineurin/CN) bind substrates and regulatory proteins using short functional sequences known as Short Linear Motifs (SLiMs).
Xinru Wang, Rebecca Page, Peti Wolfgang
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The protein phosphatase-2A subunit PR130 is involved in the formation of cytotoxic protein aggregates in pancreatic ductal adenocarcinoma cells [PDF]

As a major source of cellular serine and threonine phosphatase activity, protein phosphatase-2A (PP2A) modulates signaling pathways in health and disease. PP2A complexes consist of catalytic, scaffolding, and B-type subunits.
Falk Butter, Alexandra Nguyen, Al-Hassan M. Mustafa, Halilovic, Melisa, Murr, Janine, Alessa K. Leydecker, Krämer, Oliver H., Oliver H. Krämer, Nguyen, Alexandra, Leydecker, Alessa K., Butter, Falk, Melisa Halilovic, Janine Murr, Mustafa, Al-Hassan M., Nguyen, Alexandra ; Mustafa, Al-Hassan M. ; Leydecker, Alessa K. ; Halilovic, Melisa ; Murr, Janine ; Butter, Falk ; Krämer, Oliver H.   +14 more
core   +1 more source