Results 51 to 60 of about 6,324,586 (257)

Proteasomal degradation competes with Mia40-mediated import into mitochondria

BMC Biology, 2018
Tandem fluorescent protein timers are elegant tools to determine proteolytic stabilities of cytosolic proteins with high spatial and temporal resolution. In a new study published in BMC Biology, Kowalski et al.
Eva Zöller, R. Todd Alexander, Johannes M. Herrmann   +2 more
doaj   +1 more source

Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors

Scientific Reports, 2022
The Cre-loxP system has been widely used for cell- or organ-specific gene manipulation, but it is important to precisely understand what kind of cells the recombination takes place in.
Shinya Ikeda, Sachiko Sugioka, Takeshi Kimura, Noboru Ashida   +3 more
doaj   +1 more source

Fast Proteome Identification and Quantification from Data-Dependent Acquisition - Tandem Mass Spectrometry using Free Software Tools [PDF]

Methods and Protocols 2019, 2018
Identification of nearly all proteins in a system using data-dependent acquisition (DDA) mass spectrometry has become routine for simple organisms, such as bacteria and yeast. Still, quantification of the identified proteins may be a complex process and require multiple different software packages.
arxiv   +1 more source

Escherichia coli SecB protein associates with exported protein precursors in vivo.

Proceedings of the National Academy of Sciences of the United States of America, 1989
The product of the Escherichia coli secB gene is required for efficient export of proteins across the cytoplasmic membrane. The studies described in this report show that in wild-type growing cells, SecB protein associates with precursor forms of ...
C. Kumamoto
semanticscholar   +1 more source

Motor recruitment to the TIM23 channel’s lateral gate restricts polypeptide release into the inner membrane

Nature Communications, 2018
The mitochondrial TIM23-complex facilitates anterograde precursor transport across the inner membrane into the matrix and lateral release of precursors into the membrane.
Alexander Benjamin Schendzielorz, Piotr Bragoszewski, Nataliia Naumenko, Ridhima Gomkale, Christian Schulz, Bernard Guiard, Agnieszka Chacinska, Peter Rehling   +7 more
doaj   +1 more source

Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.

PLoS ONE, 2021
In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors ...
Gisela Pöll, Michael Pilsl, Joachim Griesenbeck, Herbert Tschochner, Philipp Milkereit   +4 more
doaj   +1 more source

Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy

Bio-Protocol, 2021
Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal ...
Salvatore Scaffidi, Mac Shebes, Wenqi Yu
doaj   +1 more source

Androgens affect the processing of secretory protein precursors in the guinea pig seminal vesicle. II. Identification of conserved sites for protein processing.

Molecular Endocrinology, 1989
The guinea pig seminal vesicle epithelium is an androgen-dependent tissue that synthesizes and secretes four major secretory proteins (SVP-1, SVP-2, SVP-3, and SVP-4).
J. Hagstrom, S. Harvey, B. Madden, D. Mccormick, E. Wieben   +4 more
semanticscholar   +1 more source

Elovanoids downregulate SARS-CoV-2 cell-entry, canonical mediators and enhance protective signaling in human alveolar cells

Scientific Reports, 2021
The pro-homeostatic lipid mediators elovanoids (ELVs) attenuate cell binding and entrance of the SARS-CoV-2 receptor-binding domain (RBD) as well as of the SARS-CoV-2 virus in human primary alveoli cells in culture.
Jorgelina M. Calandria, Surjyadipta Bhattacharjee, Nicholas J. Maness, Marie-Audrey I. Kautzmann, Aram Asatryan, William C. Gordon, Khanh V. Do, Bokkyoo Jun, Pranab K. Mukherjee, Nicos A. Petasis, Nicolas G. Bazan   +10 more
doaj   +1 more source

Androgens affect the processing of secretory protein precursors in the guinea pig seminal vesicle. I. Evidence for androgen-regulated proteolytic processing.

Molecular Endocrinology, 1989
The guinea pig seminal vesicle epithelium synthesizes and secretes four major secretory proteins (SVP-1-4). Previous work has established that these four proteins are cleaved from two primary translation products in a complex series of protein processing
M. Norvitch, S. Harvey, S. Smith, J. Hagstrom, E. Wieben   +4 more
semanticscholar   +1 more source