Results 231 to 240 of about 5,630,720 (303)

Two-Step Engineering of Food-Grade <i>Aspergillus oryzae</i> via Endogenous Signal Peptides and Vesicle Trafficking Proteins to Enhance Carrier-Free Protein Secretion. [PDF]

J Fungi (Basel)
Panchanawaporn S, Rattanaphan N, Jeennor S, Anantayanon J, Woraprayote W, Pumpuang L, Karirat T, Prompakdee N, Laoteng K, Chutrakul C.   +9 more
europepmc   +1 more source

Defined Matrices Reveal Epithelial‐ and Mesenchymal‐Specific Responses to Stiffness and Adhesion in Human iPSC‐Derived Intestinal Organoids

Advanced Materials, EarlyView.
Defined PEG hydrogels enable human iPSC‐derived intestinal organoid culture without Matrigel. PEG‐ and Matrigel‐cultured organoids both support duodenal‐like epithelial identity, but direct comparison reveals subtle epithelial differentiation‐marker shifts and stronger mesenchymal ECM‐remodeling/integrin programs in PEG.
Alicja Kuziola, Tamara Fawzi, Emma H. Kromann, Marion Leleu, Joanna M. Drelinkiewicz, Dusko Ilic, Cécile A. Dreiss, Joana F. Neves, Eileen Gentleman   +8 more
wiley   +1 more source

YTK Display-and-Secrete: Screening for Optimal Protein Secretion Elements in <i>Saccharomyces cerevisiae</i>. [PDF]

ACS Synth Biol
Kishkevich A, Ciurkot K, Ellis T.
europepmc   +1 more source

A Coaxial Bioprinted Hydrogel With Semipermeable Sheath and Pro‐Chondrogenic Core for Articular Cartilage Repair

Advanced Materials, EarlyView.
A sheath‐core biomimetic hydrogel is engineered with a compartmentalized yet integrated architecture for synergistic multifunctionality. Its stiff, semipermeable sheath mimics the ECM's load‐bearing and protective roles, encapsulating a soft core that provides a pro‐chondrogenic microenvironment similar to the PCM.
Zheyuan Shi, Hua Zhang, Jingying Hu, Guanrong Li, Dianqing Wang, Cuicui Su, Gordon G. Wallace, Qi Gu, Jun Chen, Yingchun Zhu   +9 more
wiley   +1 more source

Norovirus co-opts NINJ1 for selective protein secretion. [PDF]

Sci Adv
Song J, Zhang L, Moon S, Fang A, Wang G, Gheshm N, Loeb SA, Cao P, Wallace JR, Alfajaro MM, Strine MS, Beatty WL, Jamieson AM, Orchard RC, Robinson BA, Nice TJ, Wilen CB, Orvedahl A, Reese TA, Lee S.   +19 more
europepmc   +1 more source

An Engineered Thio‐Amino Polymer Achieves Potent, Safe, and Broad Cell Type Delivery in the Lung Mapped by Spatial Transcriptomics

Advanced Materials, EarlyView.
Synthesis and screening of a polymer library produced a versatile and inhalable mRNA vehicle for the lungs (P81). Compared to a published LNP, P81 provides increased delivery efficacy, achieving widespread expression in broad cell types, with no toxicity.
Pramodkumar EK, Younghun Jung, Elex S. Rose, Jae Yeon Joo, James R. Rose, José A. F. Assumpção, Hyeyoon Jeong, Lorena C. S. Chaves, Blake Andrew Miller, João Paulo Pera Mendes, Hannah E. Peck, Charmy J. Patel, Loren E. Sasser, Heather Pathak, Varun V. Mosur, Jaehyeon Hwang, Guilherme de Oliveira Lopes, Merrilee Thoresen, Santiago Cornejo, Peres Badial, Alicia Olivier, Amelia R. Woolums, Samuele Stazzoni, Noemi Manganaro, Eleonora Nencini, Giulio Pierleoni, Emanuele Andreano, Rino Rappuoli, Nichole Orr‐Burks, Sarah R. Hosking, Nicholas S. Heaton, Eric R. Lafontaine, Robert J. Hogan, Francois Villinger, Daryll Vanover, Chiara Zurla, Philip J. Santangelo   +36 more
wiley   +1 more source

Screening signal peptides from Bacillus thuringiensis secretome for heterologous protein secretion. [PDF]

Appl Microbiol Biotechnol
Yang J, Xia Y, Zhu L, Wang Z, Song F, Guo S.   +5 more
europepmc   +1 more source

A U‐shaped Microfluidic Platform for Culturing Vascularized Colorectal Cancer Spheroids Reveals Tumor–Endothelial Crosstalk and Invasion‐Associated Remodeling

Advanced Materials Technologies, EarlyView.
A closed U‐shaped microfluidic platform symmetrically embeds a single, size‐controlled HT29 colorectal cancer spheroid within a GFP‐HUVEC‐laden fibrin matrix. HT29 spheroids are associated with hyper‐branched endothelial network remodeling, while vascularized co‐culture is accompanied by anisotropic protrusive growth and EMT‐like marker changes in the ...
G. Cretti, M. Ballerini, A. Mantegazza, P. Occhetta, M. Rasponi   +4 more
wiley   +1 more source

Human dorsal forebrain organoids show differentiation-state-specific protein secretion. [PDF]

iScience
Yentür Z, Kagermeier T, Sarieva K, Jarboui MA, Becker K, Mayer S.   +5 more
europepmc   +1 more source

A Human Bone Defect‐on‐a‐Chip Model for Studying Bone Regeneration

Advanced Materials Technologies, EarlyView.
This study presents a novel human bone defect‐on‐a‐chip (BDOC) model, aiming to recapitulate key processes during early bone healing, incorporating bone graft substitutes. Over 21 days, human mesenchymal stem/stromal cells (HMSCs) and human primary macrophages are co‐cultured in the BDOC together with calcium phosphate microparticles.
Maria Gabriella Fois, Zeinab N. Tahmasebi Birgani, Francesca Giacomini, Alexander P. M. Guttenplan, David Baião Barata, Hoon Suk Rho, Noel Davison, Clemens van Blitterswijk, Martijn van Griensven, Stefan Giselbrecht, Pamela Habibović, Roman K. Truckenmüller   +11 more
wiley   +1 more source