Results 31 to 40 of about 6,974,231 (359)
Microbial Cell Factories, 2019 Background The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to ...
Lukas Marsalek +4 more
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Non-classical protein secretion in bacteria
BMC Microbiology, 2005 Background We present an overview of bacterial non-classical secretion and a prediction method for identification of proteins following signal peptide independent secretion pathways.
Fausbøll Anders +3 more
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OutCyte: a novel tool for predicting unconventional protein secretion
Scientific Reports, 2019 The prediction of protein localization, such as in the extracellular space, from high-throughput data is essential for functional downstream inference.
Linlin Zhao +5 more
semanticscholar +1 more source
Analysis of Mycobacterial Protein Secretion
Bio-Protocol, 2014 Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis. Analysis of proteins secreted by Mtb has been of interest to the field of tuberculosis research since certain secreted proteins interact with the host to promote virulence, while ...
Alka Mehra, Jennifer Philips
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Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins
Frontiers in Microbiology, 2018 Fluorescent proteins are a major cell biology tool to analyze protein sub-cellular topology. Here we have applied this technology to study protein secretion in the Gram-positive bacterium Streptomyces lividans TK24, a widely used host for heterologous ...
Mohamed Belal Hamed +12 more
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Frontiers in Bioengineering and Biotechnology, 2019 Signal peptides are short peptides directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences are ubiquitous to all prokaryotes and eukaryotes.
Chong Peng +5 more
semanticscholar +1 more source
Microbial Cell Factories, 2017 Background The gene encoding a thermostable cellulase of family 12 was previously isolated from a Rhodothermus marinus through functional screening. CelA is a protein of 260 aminoacyl residues with a 28-residue amino-terminal signal peptide.
Mohamed Belal Hamed +12 more
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Single-Cell Protein Secretion Detection and Profiling.
Annual Review of Analytical Chemistry, 2019 Secreted proteins play important roles in mediating various biological processes such as cell-cell communication, differentiation, migration, and homeostasis at the population or tissue level.
Zhuo Chen, Jonathan J. Chen, R. Fan
semanticscholar +1 more source
Molecular Plant-Microbe Interactions, 2000 Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis.
Suvendra K. Ray +2 more
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Signal peptides for recombinant protein secretion in bacterial expression systems
Microbial Cell Factories, 2018 The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into the culture supernatant of a bacterial expression host greatly facilitates their downstream processing and significantly reduces the production costs.
R. Freudl
semanticscholar +1 more source