Developing Gram-negative bacteria for the secretion of heterologous proteins
Microbial Cell Factories, 2018 Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large cultures. However, the utility of these microbes would expand if they also could secrete the
Lisa Ann Burdette +3 more
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Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
mBio, 2022 Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes.
Rory Hennell James +4 more
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Environmental salinity determines the specificity and need for tat-dependent secretion of the YwbN protein in bacillus subtilis [PDF]
, 2011 Twin-arginine protein translocation (Tat) pathways are required for transport of folded proteins across bacterial, archaeal and chloroplast membranes. Recent studies indicate that Tat has evolved into a mainstream pathway for protein secretion in certain
Miethke, Marcus +45 more
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Biomedicines, 2022 The NF-κB system is a key transcriptional pathway that regulates innate and adaptive immunity because it triggers the activation and differentiation processes of lymphocytes and myeloid cells during immune responses.
Jasmin Priester +3 more
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Loss of AMP-activated protein kinase alpha 2 subunit in mouse beta-cells impairs glucose-stimulated insulin secretion and inhibits their sensitivity to hypoglycaemia [PDF]
, 2010 AMPK (AMP-activated protein kinase) signalling plays a key role in whole-body energy homoeostasis, although its precise role in pancreatic beta-cell function remains unclear.
Smith, Mark A. +11 more
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Nature Communications, 2019 Reliable methods of measuring intracellular H2O2 fluctuations are necessary to advance redox biology. Here the authors design a H2O2 sensor based on the fission yeast peroxiredoxin Tpx1 to sense nanomolar fluctuations of intracellular H2O2 in response to
Mercè Carmona +8 more
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Microreview: type IV secretion systems: versatility and diversity in function [PDF]
, 2010 Type IV secretion systems (T4SSs) are large protein complexes which traverse the cell envelope of many bacteria. They contain a channel through which proteins or protein–DNA complexes can be translocated.
Angel Rivera-Calzada +5 more
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mBio, 2017 Sophisticated nanomachines are used by bacteria for protein secretion. In Gram-negative bacteria, the type 2 secretion system (T2SS) is composed of a pseudopilus assembly platform in the inner membrane and a secretin complex in the outer membrane.
Iain D. Hay +2 more
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Dual Targeting Factors Are Required for LXG Toxin Export by the Bacterial Type VIIb Secretion System
mBio, 2022 Bacterial type VIIb secretion systems (T7SSb) are multisubunit integral membrane protein complexes found in Firmicutes that play a role in both bacterial competition and virulence by secreting toxic effector proteins.
Timothy A. Klein +7 more
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CagI is an essential component of the Helicobacter pylori Cag type IV secretion system and forms a complex with CagL. [PDF]
, 2012 Helicobacter pylori, the causative agent of type B gastritis, peptic ulcers, gastric adenocarcinoma and MALT lymphoma, uses the Cag type IV secretion system to induce a strong proinflammatory response in the gastric mucosa and to inject its effector ...
Haas, Rainer +17 more
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