Results 31 to 40 of about 5,630,720 (303)
Microbial Cell Factories, 2019 Background The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to ...
Lukas Marsalek +4 more
doaj +1 more source
Cell Reports, 2022 Summary: Protein machines undergo conformational motions to interact with and manipulate polymeric substrates. The Sec translocase promiscuously recognizes, becomes activated, and secretes >500 non-folded preprotein clients across bacterial cytoplasmic ...
Srinath Krishnamurthy +10 more
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Protein secretion in streptomycetes [PDF]
FEMS Microbiology Letters, 2006 Some aspects of the current knowledge on protein secretion in streptomycetes are presented including recent data on the identification of genes in the general secretory pathway, on the importance of the signal peptide structure and on the number of ribosome-binding sites inside signal peptides which can influence the production level of a gene product.
R, Morosoli, F, Shareck, D, Kluepfel
openaire +2 more sources
Analysis of Mycobacterial Protein Secretion
Bio-Protocol, 2014 Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis. Analysis of proteins secreted by Mtb has been of interest to the field of tuberculosis research since certain secreted proteins interact with the host to promote virulence, while ...
Alka Mehra, Jennifer Philips
doaj +1 more source
Non-classical protein secretion in bacteria
BMC Microbiology, 2005 Background We present an overview of bacterial non-classical secretion and a prediction method for identification of proteins following signal peptide independent secretion pathways.
Fausbøll Anders +3 more
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mBio, 2020 Pathogenic mycobacteria encounter multiple environments during macrophage infection. Temporally, the bacteria are engulfed into the phagosome, lyse the phagosomal membrane, and interact with the cytosol before spreading to another cell. Virulence factors
Kevin G. Sanchez +6 more
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A PASS for protein secretion. [PDF]
Nat Chem Biol, 2023 Traditional production of therapeutic secretory proteins often experiences delays between protein synthesis and therapeutic effects. An inducible protease-dependent protein secretion technique allows the immediate secretion of pre-translated biotherapeutic agents after exposure to chemical cues, tumor-specific antigens or photons.
Wang T, Zhou Y.
europepmc +4 more sources
Prediction of type III secretion signals in genomes of gram-negative bacteria [PDF]
, 2009 Background: Pathogenic bacteria infecting both animals as well as plants use various mechanisms to transport virulence factors across their cell membranes and channel these proteins into the infected host cell.
Löwer, Martin +5 more
core +2 more sources
Distribution and diversity of bacterial secretion systems across metagenomic datasets [PDF]
, 2012 Bacteria can manipulate their surrounding environment through the secretion of proteins into other living organisms and into the extracellular milieu. In Gram stain negative bacteria this process is mediated by different types of secretion systems from ...
Egan, Frank +3 more
core +2 more sources
Monitoring Protein Secretion in Streptomyces Using Fluorescent Proteins
Frontiers in Microbiology, 2018 Fluorescent proteins are a major cell biology tool to analyze protein sub-cellular topology. Here we have applied this technology to study protein secretion in the Gram-positive bacterium Streptomyces lividans TK24, a widely used host for heterologous ...
Mohamed Belal Hamed +12 more
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