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Stabilization of Proteins for Storage

Cold Spring Harbor Protocols, 2010
INTRODUCTIONFollowing isolation and purification, it is often necessary to store proteins and peptides for extended periods of time before performing detailed biophysical, enzymatic, and structural proteomics. Therefore, it is essential that the pure target protein maintain its original biological (or functional) behavior over an extended period of ...
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Stabilization of protein structures

Current Opinion in Biotechnology, 1997
The technique of protein stabilization has been improving steadily in recent years, but it is only in the past year or two that the stability of some protein molecules has been improved to the level of those from extreme thermophilic organisms. This was achieved by multiple mutations and often by utilizing the knowledge gained from the homologous ...
B, Lee, G, Vasmatzis
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Pressure Stability of Proteins

Annual Review of Physical Chemistry, 1993
The stability of proteins toward temperature has been explored in much detail since the time that proteins were characterized as chemicals of constant composition, but the study of the effects of pressure upon pro­ teins is much more recent and has been much less frequent than that of temperature.
J L, Silva, G, Weber
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Stability of crystalline proteins

Biotechnology and Bioengineering, 2001
AbstractBy using two model proteins, glucose oxidase and lipase, we demonstrate that dry crystalline formulations are significantly more stable than their amorphous counterparts. The results of Fourier‐transform infrared spectroscopy indicate that crystalline proteins better maintain their native conformation in accelerated stability studies. The lower
B, Shenoy   +3 more
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Thermal Stability of Proteins

Annals of the New York Academy of Sciences, 2006
Abstract: Protein stability is critical to the outcome of nearly all thermally mediated applications to biomaterials such as thermal therapies (including cryosurgery), burn injury, and biopreservation. As such, it is imperative to understand as much as possible about how a protein loses stability and to what extent we can control this through the ...
John C, Bischof, Xiaoming, He
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The Stability of Globular Protein

CRC Critical Reviews in Biochemistry, 1975
(1975). The Stability of Globular Protein. CRC Critical Reviews in Biochemistry: Vol. 3, No. 1, pp. 1-43.
C. N. Pace, Jan Hermans
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Destabilization and stabilization of proteins

Quarterly Reviews of Biophysics, 2005
Introduction 351Part I. A succession of concepts 3521. Cooperativity 3522. Cosolvent interaction 3523. Linearity 3544. Solvent exchange 3545. Excluded volume 3566. Summation 357Part II 1. The Kirkwood–Buff approach 357Acknowledgments 360References 360In Part I the history of progress in the stabilization and destabilization of protein conformations by ...
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Mutations and protein stability

Biopolymers, 1981
AbstractThe comparative study of proteins which differ in primary structure by point mutations permits one to use thermodynamic experiments to obtain information about the role of specific amino acids in determining protein structure and stability. We have now determined the thermodynamic changes induced in six mutants of T4 lysozyme and have compared ...
J A, Schellman   +3 more
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Protein-stabilized emulsions

Journal of Food Engineering, 1994
Abstract Factors affecting the structure and composition of adsorbed protein layers are described with reference to macroscopic oil-water interfaces and protein-stabilized oil-in-water emulsions. Measurements of specular neutron reflectance and surface shear viscosity provide information on differences in adsorbed layers between the disordered β ...
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Engineering Protein Stability

2010
This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004-2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation studies and from study of thermophilic (and other extremophilic) proteins.
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