Results 61 to 70 of about 12,579,753 (296)

Arp2/3 Inhibition Synergizes With PARP Inhibitors by Impairing Homologous Recombination in Gastric Cancer Cells

Frontiers in Bioscience-Landmark
Background:Poly(ADP-ribose) polymerase inhibitors (PARPi) have shown limited efficacy in gastric cancer, primarily due to the rarity of inherent homologous recombination (HR) deficiency.
Wenlong Li, Xinyue Pei, Yongpei Li, Yanji Lu, Xian Hong, Ming Sui, Qiang Li, Xueying Sun, Jingqiu Wang, Enming Zhang, Yihan Liu, Tao Wang, Jing Xu, Zhihui Deng   +13 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Molecular insights into Spindlin1-HBx interplay and its impact on HBV transcription from cccDNA minichromosome

Nature Communications, 2023
Molecular interplay between host epigenetic factors and viral proteins constitutes an intriguing mechanism for sustaining hepatitis B virus (HBV) life cycle and its chronic infection.
Wei Liu, Qiyan Yao, Xiaonan Su, Yafang Deng, Mo Yang, Bo Peng, Fan Zhao, Chao Du, Xiulan Zhang, Jinsong Zhu, Daliang Wang, Wenhui Li, Haitao Li   +12 more
doaj   +1 more source

Protein Structural Phylogenetics

Genome Biology and Evolution
Abstract Protein structural phylogenetics is an interdisciplinary branch of molecular evolution that (i) uses 3D structural data to trace evolutionary histories, and (ii) uses these evolutionary relationships to explore the diversity of protein structures and their ancestral functions. The appeal in extracting phylogenetic information
Caroline Puente-Lelievre, Ashar Malik, Jordan Douglas   +2 more
openaire   +4 more sources

The Structural Protein of Epidermis

Journal of Investigative Dermatology, 1972
These studies suggest a scheme of keratinization which is unique for epidermal tissue and different from hair and nail. Prekeratin appears to be formed as synthesis of the peptides proceeds or soon after release from the ribosomes. No neutral-soluble precursor in this process can be identified. These newly formed molecules, consisting of 2 or 3 peptide
Baden, Howard P, Goldsmith, Lowell A
openaire   +2 more sources

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Wnt signaling polarizes cortical actin polymerization to increase daughter cell asymmetry

Cell Discovery, 2022
Asymmetric positioning of the mitotic spindle contributes to the generation of two daughter cells with distinct sizes and fates. Here, we investigated an asymmetric division in the Caenorhabditis elegans Q neuroblast lineage.
Yongping Chai, Dong Tian, Zhiwen Zhu, Yuxiang Jiang, Shanjin Huang, Dou Wu, Guangshuo Ou, Wei Li   +7 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Macromolecular crystallography from an industrial perspective – the impact of synchrotron radiation on structure-based drug discovery

Journal of Synchrotron Radiation
Structure-based drug design has been an integral part of drug discovery for over three decades, contributing to the development of numerous approved drugs. Here we discuss the evolution, as well as the current state, of structure-based drug design within
H. Käck, T. Sjögren
doaj   +1 more source