Structure of the Saccharolobus solfataricus type III-D CRISPR effector
Current Research in Structural Biology, 2023 CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a ...
Giuseppe Cannone +4 more
doaj +1 more source
Subunit Exchange in Protein Complexes
Journal of Molecular Biology, 2018 Over the past 50 years, protein complexes have been studied with techniques such as X-ray crystallography and electron microscopy, generating images which although detailed are static and homogeneous. More recently, limited application of in vivo fluorescence and other techniques has revealed that many complexes previously thought stable and ...
Tusk, S, Delalez, N, Berry, R
openaire +2 more sources
Mitochondrial Protein Import [PDF]
, 1987 The role of nucleoside triphosphates (NTPs) in mitochondrial protein import was investigated with the precursors of N. crassa ADP/ATP carrier, F1-ATPase subunit β, F0-ATPase subunit 9, and fusion proteins between subunit 9 and mouse dihydrofolate ...
Arends +72 more
core +1 more source
The role of TcdB and TccC subunits in secretion of the photorhabdus Tcd toxin complex [PDF]
, 2013 The Toxin Complex (TC) is a large multi-subunit toxin encoded by a range of bacterial pathogens. The best-characterized examples are from the insect pathogens Photorhabdus, Xenorhabdus and Yersinia.
A Sisto +37 more
core +3 more sources
Interaction between the assembly of the ribosomal subunits: Disruption of 40S ribosomal assembly causes accumulation of extra-ribosomal 60S ribosomal protein uL18/L5. [PDF]
PLoS ONE, 2020 Inhibition of the synthesis of an essential ribosomal protein (r-protein) abrogates the assembly of its cognate subunit, while assembly of the other subunit continues.
Nusrat Rahman +2 more
doaj +1 more source
How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism. [PDF]
PLoS ONE, 2011 Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements.
Chao-Sheng Chen +6 more
doaj +1 more source
Computational modeling of TC0583 as a putative component of the Chlamydia muridarum V-type ATP synthase complex and assessment of its protective capabilities as a vaccine antigen. [PDF]
, 2016 Numerous Chlamydia trachomatis proteins have been identified as potential subunit vaccines, of which the major outer-membrane protein (MOMP) has, so far, proven the most efficacious. Recently, subunit A of the V-type ATP synthase (ATPase; TC0582) complex
Barta, Michael L +4 more
core +1 more source
Biogenesis of mitochondrial ubiquinol [PDF]
, 1982 The precursor proteins to the subunits of ubiquinol:cytochrome c reductase (cytochrome bc1 complex) of Neurospora crassa were synthesized in a reticulocyte lysate.
Neupert, Walter +3 more
core +1 more source
Scientific Reports, 2023 We developed a new method to analyze protein–protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein–protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fragment (epitope ...
Agnieszka M. Murakami +5 more
doaj +1 more source
Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans [PDF]
, 1991 A cDNA corresponding to a known G protein alpha subunit, the alpha subunit of Go (Go alpha), was isolated and sequenced. The predicted amino acid sequence of C. elegans Go alpha is 80-87% identical to other Go alpha sequences.
Lochrie, Michael A. +3 more
core +1 more source