Protein Unfolding-Thermodynamic Perspectives and Unfolding Models. [PDF]
Int J Mol Sci, 2023 AbstractProtein unfolding is a dynamic cooperative process with many short-lived intermediates. Cooperativity means that similar molecular elements act dependently on each other. The thermodynamics of protein unfolding can be determined with differential scanning calorimetry (DSC).
Seelig J, Seelig A.
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Engineering shape memory and morphing protein hydrogels based on protein unfolding and folding. [PDF]
Nat Commun, 2022 Engineering shape memory/morphing materials have achieved considerable progress in polymer-based systems with broad potential applications. However, engineering protein-based shape memory/morphing materials remains challenging and under-explored. Here we
Bian Q, Fu L, Li H.
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A General Protein Unfolding-Chemical Coupling Strategy for Pure Protein Hydrogels with Mechanically Strong and Multifunctional Properties. [PDF]
Adv Sci (Weinh), 2022 Protein‐based hydrogels have attracted great attention due to their excellent biocompatible properties, but often suffer from weak mechanical strength.
Tang Z +10 more
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Protein Unfolding and Aggregation near a Hydrophobic Interface. [PDF]
Polymers (Basel), 2021 The behavior of proteins near interfaces is relevant for biological and medical purposes. Previous results in bulk show that, when the protein concentration increases, the proteins unfold and, at higher concentrations, aggregate.
March D, Bianco V, Franzese G.
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Determining suitable surfactant concentration ranges to avoid protein unfolding in pharmaceutical formulations using UV analysis [PDF]
Heliyon, 2023 Protein stability is fundamental to maintain pharmaceutical efficacy in the nascent field of biologics. One particular property that is essential for therapeutic effect is retention of the folded 3-dimensional conformation, i.e.
Laura J. Waters +3 more
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Protein Unfolding: Denaturant vs. Force [PDF]
Biomedicines, 2021 While protein refolding has been studied for over 50 years since the pioneering work of Christian Anfinsen, there have been a limited number of studies correlating results between chemical, thermal, and mechanical unfolding.
Colleen Kelly, Matthew J. Gage
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Characterization of protein unfolding by fast cross-linking mass spectrometry using di-ortho-phthalaldehyde cross-linkers. [PDF]
Nat Commun, 2022 Chemical cross-linking of proteins coupled with mass spectrometry is widely used in protein structural analysis. In this study we develop a class of non-hydrolyzable amine-selective di-ortho-phthalaldehyde (DOPA) cross-linkers, one of which is called ...
Wang JH +13 more
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Control of Nanoscale <i>In Situ</i> Protein Unfolding Defines Network Architecture and Mechanics of Protein Hydrogels. [PDF]
ACS Nano, 2021 Hierarchical assemblies of proteins exhibit a wide-range of material properties that are exploited both in nature and by artificially by humankind. However, little is understood about the importance of protein unfolding on the network assembly, severely ...
Hughes MDG +5 more
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A modular DNA origami nanocompartment for engineering a cell-free, protein unfolding and degradation pathway. [PDF]
Nat NanotechnolWithin the cell, chemical reactions are often confined and organized through a modular architecture. This facilitates the targeted localization of molecular species and their efficient translocation to subsequent sites.
Huang J +8 more
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Protein unfolding by SDS: the microscopic mechanisms and the properties of the SDS-protein assembly. [PDF]
Nanoscale, 2020 The effects of detergent sodium dodecyl sulfate (SDS) on protein structure and dynamics are fundamental to the most common laboratory technique used to separate proteins and determine their molecular weights: polyacrylamide gel electrophoresis.
Winogradoff D, John S, Aksimentiev A.
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