Results 71 to 80 of about 461,354 (348)

DART-ID increases single-cell proteome coverage. [PDF]

, 2019
Analysis by liquid chromatography and tandem mass spectrometry (LC-MS/MS) can identify and quantify thousands of proteins in microgram-level samples, such as those comprised of thousands of cells.
Chen, Albert Tian, Franks, Alexander, Slavov, Nikolai   +2 more
core   +2 more sources

Integration of Human Protein Sequence and Protein-Protein Interaction Data by Graph Autoencoder to Identify Novel Protein-Abnormal Phenotype Associations

Cells, 2022
Understanding gene functions and their associated abnormal phenotypes is crucial in the prevention, diagnosis and treatment against diseases. The Human Phenotype Ontology (HPO) is a standardized vocabulary for describing the phenotype abnormalities ...
Yuan Liu, Ruirui He, Yingjie Qu, Yuan Zhu, Dianke Li, Xinping Ling, Simin Xia, Zhenqiu Li, Dong Li   +8 more
doaj   +1 more source

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

Journal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
arxiv   +1 more source

Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition

Nature Biotechnology
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes in a fast and reproducible manner. Here we present the narrow-window data-independent acquisition (nDIA) strategy consisting of high-resolution MS1 scans with parallel ...
Ulises H. Guzmán, Ana Martínez-Val, Zilu Ye, Eugen Damoc, T. Arrey, A. Pashkova, Santosh Renuse, E. Denisov, J. Petzoldt, Amelia C. Peterson, Florian Harking, Ole Østergaard, Rasmus Rydbirk, Susana Aznar, H. Stewart, Y. Xuan, Daniel Hermanson, S. Horning, Christian Hock, Alexander A. Makarov, V. Zabrouskov, J. Olsen   +21 more
semanticscholar   +1 more source

Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation

bioRxiv, 2020
Single-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing1–3. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS ...
Andreas-David Brunner, Marvin Thielert, Catherine G. Vasilopoulou, Constantin Ammar, F. Coscia, A. Mund, Ole Hoerning, N. Bache, Amalia Apalategui, M. Lubeck, Sabrina Richter, David S. Fischer, O. Raether, Melvin A Park, F. Meier, Fabian J Theis, M. Mann   +16 more
semanticscholar   +1 more source

Peripheral blood proteome biomarkers distinguish immunosuppressive features of cancer progression

Molecular Oncology, EarlyView.
Immune status significantly influences cancer progression. This study used plasma proteomics to analyze benign 67NR and malignant 4T1 breast tumor models at early and late tumor stages. Immune‐related proteins–osteopontin (Spp1), lactotransferrin (Ltf), calreticulin (Calr) and peroxiredoxin 2 (Prdx2)–were associated with systemic myeloid‐derived ...
Yeon Ji Park, Jae Won Oh, Hyewon Chung, Jung Won Kwon, Yi Rang Na, Kwang Pyo Kim, Seung Hyeok Seok   +6 more
wiley   +1 more source

Sex-partitioning of the Plasmodium falciparum stage V gametocyte proteome provides insight into falciparum-specific cell biology [PDF]

, 2014
One of the critical gaps in malaria transmission biology and surveillance is our lack of knowledge about Plasmodium falciparum gametocyte biology, especially sexual dimorphic development and how sex ratios that may influence transmission from the human ...
Dinglasan, R. R., Goldowitz, I., Graham, D. R., King, J. G., Marti, M., Mathias, D. K., Moss, E., Pastrana-Mena, R., Tao, D., Tripathi, A., Ubaida-Mohien, C.   +10 more
core   +1 more source

Stable expression of a truncated TLX variant drives differentiation of induced pluripotent stem cells into self-renewing neural stem cells for production of extracellular vesicles

Stem Cell Research & Therapy, 2022
Background Neural stem cells (NSCs)-derived extracellular vesicles (EVs) possess great potential in treating severe neurological and cerebrovascular diseases, as they carry the modulatory and regenerative ingredients of NSCs.
Mingzhi Xu, Gang Chen, Yanan Dong, Shensi Xiang, Miaomiao Xue, Yongxue Liu, Haijing Song, Haifeng Song, Yi Wang   +8 more
doaj   +1 more source

The human protein atlas: A spatial map of the human proteome

Protein Science, 2018
The correct spatial distribution of proteins is vital for their function and often mis‐localization or ectopic expression leads to diseases. For more than a decade, the Human Protein Atlas (HPA) has constituted a valuable tool for researchers studying ...
Peter J. Thul, C. Lindskog
semanticscholar   +1 more source

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Elevated level of cholesterol is positively correlated to prostate cancer development and disease severity. Cholesterol‐lowering drugs, such as statins, are demonstrated to inhibit prostate cancer. VNPP433‐3β interrupts multiple signaling and metabolic pathways, including cholesterol biosynthesis, AR‐mediated transcription of several oncogenes, mRNA 5′
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
wiley   +1 more source