Current Protocols in Protein Science, 2006 AbstractThis unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided.
Lescuyer, Pierre +3 more
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An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes. [PDF]
, 2018 Mass spectrometry (MS) has become a crucial technique for the analysis of protein complexes. Native MS has traditionally examined protein subunit arrangements, while proteomics MS has focused on sequence identification.
Campuzano, Iain DG +4 more
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Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
, 2009 Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi +103 more
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Sparse Proteomics Analysis - A compressed sensing-based approach for feature selection and classification of high-dimensional proteomics mass spectrometry data [PDF]
, 2016 Background: High-throughput proteomics techniques, such as mass spectrometry (MS)-based approaches, produce very high-dimensional data-sets. In a clinical setting one is often interested in how mass spectra differ between patients of different classes ...
Conrad, Tim +7 more
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Quantitative proteomics in resected renal cancer tissue for biomarker discovery and profiling [PDF]
, 2014 <b>Background:</b> Proteomics-based approaches for biomarker discovery are promising strategies used in cancer research. We present state-of-art label-free quantitative proteomics method to assess proteome of renal cell carcinoma (RCC ...
Atrih, A. +8 more
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Metabolites, 2022 Metabolism and aging are closely connected. The choline derivative glycerophosphocholine (GPC), an important precursor of the neurotransmitter acetylcholine, plays important roles in brain and nervous system function.
Jia-Yu Liu +11 more
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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples
Nature Communications, 2019 In-depth characterization of complex glycomes is complicated by the immense structural diversity of glycans. Here, the authors present a mass spectrometry-based strategy for untargeted, sensitive glycan profiling and identify 167 N-glycan compositions in
Guinevere S. M. Lageveen-Kammeijer +7 more
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Definition of valid proteomic biomarkers: a bayesian solution [PDF]
, 2009 Clinical proteomics is suffering from high hopes generated by reports on apparent biomarkers, most of which could not be later substantiated via validation.
C.C. Holmes +10 more
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Protein processing characterized by a gel-free proteomics approach [PDF]
, 2008 We describe a method for the specific isolation of representative N-terminal peptides of proteins and their proteolytic fragments. Their isolation is based on a gel-free, peptidecentric proteomics approach using the principle of diagonal chromatography ...
A. Staes +17 more
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Stable isotopic labeling in proteomics [PDF]
, 2008 Labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) is widely used in quantitative proteomics.
Beynon +69 more
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