Results 131 to 140 of about 904,217 (264)

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, Volume 13, Issue 49, 3 September 2026.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

Protoplast Fusion, Genetic Engineering in Higher Plants

, 1984
Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the
Sytnik, Konstantin Merkurevich, Shoeman, R., Shoeman, Robert L., Sytnik, K., Gleba, Iuri Iurevich, Gleba, I.   +5 more
core  

Nitrogen‐Starved Wheat: A Multi‐Omics Perspective From Epigenome Regulation to Grain Quality

Journal of Sustainable Agriculture and Environment, Volume 5, Issue 3, September 2026.
This review establishes epigenetic regulation, specifically H3K27ac and H3K27me3 dynamics at nitrogen transporter, assimilation, and storage protein loci, as a principal determinant of cultivar‐specific nitrogen use efficiency in hexaploid wheat, independent of DNA sequence variation, and identifies the rhizosphere microbiome as an integral but ...
Baber Ali, Zeeshan Khan, Nijat Imin
wiley   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3196-3207, September 2026.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3316-3328, September 2026.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3231-3242, September 2026.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Plant regeneration from protoplast of Brazilian citrus cultivars

Pesquisa Agropecuária Brasileira, 2000
A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in ...
GLORIA FERNANDA JANUZZI MENDES DA, MOURÃO FILHO FRANCISCO DE ASSIS ALVES, MENDES BEATRIZ MADALENA JANUZZI   +2 more
doaj  

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3458-3472, September 2026.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Optimized Cas‐SF01 gene‐editing toolbox shortens flowering timing in commercial maize inbred JING724

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3178-3180, September 2026.
The gene‐editing tool Cas‐SF01 was optimized to maximize its efficiency in maize. The Cas‐SF01‐TREX2 configuration was superior in enabling high‐purity gene mutations. This toolkit enabled commercial maize to flower seven days earlier without yield loss, thereby securing harvests and accelerating crop breeding.
Mengyuan Liu, Yao Wang, Lu Zhang, Xiang Zhang, Xiaqing Wang, Xinxiang Liu, Yue Fu, Xiaoxu Li, Ziheng Song, Ya Liu, Ronghuan Wang, Jiuran Zhao   +11 more
wiley   +1 more source

The IKU2/RLK7–MAPK–MINI3 signaling module controls endosperm cellularization timing to determine seed size in Arabidopsis

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3374-3390, September 2026.
The phosphorylation status of the WRKY transcription factor WRKY10/MINI3, controls the timing of endosperm cellularization in Arabidopsis, thus regulating final seed size, offering a molecular understanding that could inform future agricultural breeding strategies. ABSTRACT Seed size is a critical factor in determining crop yield.
Min Ni, Yuchen Qian, Tinggang Nong, Wangshu Mou, Niu Liu, La Yang, Dongdong Li, Huixia Shou, Juan Xu   +8 more
wiley   +1 more source