Results 1 to 10 of about 52,191 (164)

Mitochondrial organization in the developing proximal tubule is controlled by LRRK2 [PDF]

Nature Communications
The proximal tubule of the nephron performs energy-demanding functions such as resorption of water, amino acids and glucose. Formation of the energy-producing machinery is an essential step in proximal tubule epithelial cell differentiation, and this ...
Mohsina Khan, Kyle Bond, Elyse Grilli, Daniel Cameron, Liyang Zhao, Sunder Sims-Lucas, Andrew P. McMahon, Thomas J. Carroll, Leif Oxburgh   +8 more
doaj   +2 more sources

Comparative Analysis of Proximal Tubule Cell Sources for In Vitro Studies of Renal Proximal Tubule Toxicity [PDF]

Biomedicines
Background/Objectives: The kidneys are essential for eliminating drugs and chemicals from the human body and renal epithelial cells are particularly vulnerable to damage caused by xenobiotics and their metabolites. Drug-induced kidney toxicity is a major
Courtney Sakolish, Han-Hsuan D. Tsai, Hsing-Chieh Lin, Piyush Bajaj, Remi Villenave, Stephen S. Ferguson, Jason P. Stanko, Richard A. Becker, Philip Hewitt, Weihsueh A. Chiu, Ivan Rusyn   +10 more
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Sustained alterations in proximal tubule gene expression in primary culture associate with HNF4A loss [PDF]

Scientific Reports
Primary cultures of proximal tubule cells are widely used to model the behavior of kidney epithelial cells in vitro. However, de-differentiation of primary cells upon culture has been observed and appreciated for decades, yet the mechanisms driving this ...
Asha C. Telang, Jenna T. Ference-Salo, Madison C. McElliott, Mahboob Chowdhury, Jeffrey A. Beamish   +4 more
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Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids [PDF]

Communications Biology
Human pluripotent stem cell-derived kidney organoids are expected to be a useful tool for new drug discoveries, however, the immaturation of kidney organoids causes difficulties in recapitulating renal pharmacokinetics using organoids. Here, we performed
Yoshiki Sahara, Chie Fukui, Yuki Kuniyoshi, Minoru Takasato   +3 more
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Generation of proximal tubule spheroids for nephrotoxicity assessment [PDF]

Organoid, 2022
Background To date, nephrotoxicity in new drug development has been evaluated through two-dimensional culture of representative cell lines, such as HK-2 and human proximal tubule epithelial cells (hPTECs). Approximately 20% of new drugs that were safe in
Dae Hun Kim, Jung Hwa Lim, Cho-Rok Jung, Hyun Mi Kang   +3 more
doaj   +1 more source

Prevalence of nephropathy and proximal tubule disorder in human immunodeficiency virus patients under tenofovir therapy [PDF]

Journal of Nephropharmacology, 2022
Introduction: Tenofovir is a common therapy in human immunodeficiency virus (HIV) patients. Objective: This study was carried out to determine the prevalence of nephropathy and proximal tubule disorder in HIV patients under tenofovir therapy.
Foroogh Sabzghabaei, Afsaneh Sedighi, Raziyeh Shahi, Vahid Mahmoodi, Jahanbakhsh Khamseh, Neda Rahimian   +5 more
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Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse

Frontiers in Cell and Developmental Biology, 2023
Protein reabsorption in renal proximal tubules is essential for maintaining nutrient homeostasis. Renal proximal tubule-specific gene knockout is a powerful method to assess the function of genes involved in renal proximal tubule protein reabsorption ...
Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong, Fan Fan Hou, Fujian Zhang   +7 more
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Protein interactions with metallothionein-3 promote vectorial active transport in human proximal tubular cells

PLoS ONE, 2022
Metallothionein 3 (MT-3) is a small, cysteine-rich protein that binds to essential metals required for homeostasis, as well as to heavy metals that have the potential to exert toxic effects on cells.
Gazal Kalyan, Andrea Slusser-Nore, Jane R. Dunlevy, Chandra S. Bathula, John B. Shabb, Wallace Muhonen, Seema Somji, Donald A. Sens, Scott H. Garrett   +8 more
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Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule

Communications Biology, 2023
Of late, numerous microphysiological systems have been employed to model the renal proximal tubule. Yet there is lack of research on refining the functions of the proximal tubule epithelial layer—selective filtration and reabsorption.
Ramin Banan Sadeghian, Ryohei Ueno, Yuji Takata, Akihiko Kawakami, Cheng Ma, Toshikazu Araoka, Minoru Takasato, Ryuji Yokokawa   +7 more
doaj   +1 more source

Modelling Renal Filtration and Reabsorption Processes in a Human Glomerulus and Proximal Tubule Microphysiological System

Micromachines, 2021
Kidney microphysiological systems (MPS) serve as potentially valuable preclinical instruments in probing mechanisms of renal clearance and osmoregulation. Current kidney MPS models target regions of the nephron, such as the glomerulus and proximal tubule
Stephanie Y. Zhang, Gretchen J. Mahler
doaj   +1 more source