Results 41 to 50 of about 20,467 (222)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

open access: yesAdvanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son   +2 more
wiley   +1 more source

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

open access: yesAMB Express, 2020
The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars.
Maroua Oueslati   +8 more
doaj   +1 more source

The Diversity of Lipopeptides in the Pseudomonas syringae Complex Parallels Phylogeny and Sheds Light on Structural Diversification during Evolutionary History

open access: yesMicrobiology Spectrum, 2022
Pseudomonas spp. colonize diverse aquatic and terrestrial habitats and produce a wide variety of secondary metabolites, including lipopeptides. However, previous studies have often examined a limited number of lipopeptide-producing strains. In this study,
Alexandre Bricout   +9 more
doaj   +1 more source

Higher‐Level Structural Classification of Pseudomonas Cyclic Lipopeptides through Their Bioactive Conformation

open access: yesAdvanced Science, EarlyView.
Solution structures of a series of cyclic lipodepsipeptides from Pseudomonas reveal that these specialized metabolites adopt left‐handed alpha helical conformations of only two types, being either ‘stapled’ or ‘catch‐pole’ helix. The particular modular distribution of the biosynthetic gene clusters is found to determine the extent of their conserved ...
Benjámin Kovács   +9 more
wiley   +1 more source

Role of Jasmonic Acid Pathway in Tomato Plant-Pseudomonas syringae Interaction

open access: yesPlants, 2020
The jasmonic acid pathway has been considered as the backbone of the response against necrotrophic pathogens. However, a hemi-biotrophic pathogen, such as Pseudomonas syringae, has taken advantage of the crosstalk between the different plant hormones in ...
Loredana Scalschi   +3 more
doaj   +1 more source

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

open access: yesAdvanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han   +5 more
wiley   +1 more source

Tomato Seed-Associated Bacteria Confer Protection of Seedlings Against Foliar Disease Caused by Pseudomonas syringae

open access: yesPhytobiomes Journal, 2019
The plant microbiome is known to benefit host health in numerous ways, including providing protection against pathogens. Here, we provide evidence that tomato seed-associated microbiota play an important role in early seedling health.
Norma M. Morella   +2 more
doaj   +1 more source

Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea

open access: yesMakara Journal of Science, 2014
Lipopolysaccharide (LPS) is a major component of outer-membrane gram-negative bacteria, and it can act as a Pathogen-Associated Molecular Pattern (PAMP) for perception of pathogens by plants.
Pipit Marianingsih   +2 more
doaj   +1 more source

Pseudomonas coronafaciens sp. nov., a new phytobacterial species diverse from Pseudomonas syringae.

open access: yesPLoS ONE, 2018
We propose Pseudomonas coronafaciens sp. nov. as a new species in genus Pseudomonas, which is diverse from P. syringae. We also classified strains from onions which are responsible for yellow bud (YB) disease as P. coronafaciens.
Bhabesh Dutta   +4 more
doaj   +1 more source

Calcium signaling in plant defense

open access: yesNew Plant Protection, EarlyView.
Calcium signaling acts as a central hub in plant immunity, translating pathogen perception into defense responses. This review highlights the channels, sensors, and signaling networks involved, and discusses how pathogens subvert calcium dynamics, as well as how this knowledge may inspire innovative strategies for engineering durable crop resistance ...
Lifan Sun, Yu Wang, Jie Zhang
wiley   +1 more source

Home - About - Disclaimer - Privacy