Results 1 to 10 of about 3,673 (123)

Discrimination of psychrophilic enzymes using machine learning algorithms with amino acid composition descriptor [PDF]

Frontiers in Microbiology, 2023
IntroductionPsychrophilic enzymes are a class of macromolecules with high catalytic activity at low temperatures. Cold-active enzymes possessing eco-friendly and cost-effective properties, are of huge potential application in detergent, textiles ...
Fuping Lu, Fufeng Liu
exaly   +4 more sources

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli [PDF]

Advanced Science
Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
doaj   +3 more sources

Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12 [PDF]

Journal of Fungi, 2021
Psychrophilic organisms possess several adaptive strategies which allow them to sustain life at low temperatures between −20 to 20 °C. Studies on Antarctic psychrophiles are interesting due to the multiple stressors that exist on the permanently cold ...
Nur Athirah Yusof, Noor Haza Fazlin Hashim, Izwan Bharudin   +2 more
doaj   +2 more sources

Psychrophilic Enzymes: From Folding to Function and Biotechnology [PDF]

Scientifica, 2013
Psychrophiles thriving permanently at near-zero temperatures synthesize cold-active enzymes to sustain their cell cycle. Genome sequences, proteomic, and transcriptomic studies suggest various adaptive features to maintain adequate translation and proper
Georges Feller
doaj   +2 more sources

Some Clues about Enzymes from Psychrophilic Microorganisms

Microorganisms, 2022
Enzymes purified from psychrophilic microorganisms prove to be efficient catalysts at low temperatures and possess a great potential for biotechnological applications.
Giuseppe Graziano
exaly   +3 more sources

Discovery of a cryptic aminoacidic triad involved in the temperature adaptation of GH1 enzymes. [PDF]

FEBS J
Cold‐active enzymes retain high catalytic activity at low temperatures but are characterized by thermal instability, a behavior not fully understood. Comparison between a cold‐active GH1 and its mesophilic counterparts highlights the role of a three‐residue motif (W‐M‐F) in conferring structural stability to a mesophilic GH1.
Digiovanni S, Oanca G, Orlando M, Lotti M, Åqvist J, Mangiagalli M.   +5 more
europepmc   +2 more sources

Activation parameters, enthalpy-entropy compensation and the temperature-dependent activity of enzymes. [PDF]

Protein Sci
Abstract The increase in enzyme‐catalyzed reaction rates with temperature is typically modeled using Arrhenius or Eyring relations. Interpretation of extracted parameters is subject to multiple caveats. Here we analyze the impact of temperature variations of underlying activation or Eyring parameters and of temperature‐dependent contributions to ...
McLeod MJ, Thorne RE.
europepmc   +2 more sources

The Sensory, Chemical and Microbiological Quality of Sous-Vide Cooked Sea Bass (<i>Dicentrarchus labrax</i> Linneaus, 1758) With Liquid Smoke Flavor. [PDF]

Food Sci Nutr
The processing of sea bass fillets, flavored with liquid smoke‐salt‐vegetable oil, using sous‐vide technology is an innovative method that both enhances organoleptic quality and ensures safe preservation. The fillets, which were flavored and vacuum‐sealed, were cooked at different temperatures and then transferred to cold storage following rapid ...
Köklü R, Turan H, Altan CO, Kocatepe D, Köstekli B, Corapci B.   +5 more
europepmc   +2 more sources

Cytoplasmic fluidity and the cold life: proteome stability is decoupled from viability in psychrophiles [PDF]

Nature Communications
Protein diffusion, critical for cellular metabolism, occurs in the highly crowded cytoplasm. Understanding how this dynamics changes when organisms are adapted to different thermal niches is a fundamental challenge in microbiology and biophysics.
Beatrice Caviglia, Stepan Timr, Marianne Guiral, Marie-Thérèse Giudici-Orticoni, Tilo Seydel, Christian Beck, Judith Peters, Fabio Sterpone, Alessandro Paciaroni   +8 more
doaj   +2 more sources

Network reorganization of a cold-adapted β-glucosidase couples conformational flexibility to catalytic efficiency under low-temperature conditions [PDF]

Frontiers in Microbiology
IntroductionElucidating how cold-active enzymes maintain efficient catalysis under low-temperature conditions remains a significant question in enzymology.
Xian He, Xian He, Xian He, Mengting Liu, Mengting Liu, Mengting Liu, Xinwei Li, Xinwei Li, Xinwei Li, Liting Zheng, Liting Zheng, Liting Zheng, Derui Zhao, Derui Zhao, Derui Zhao, Peng Sang, Peng Sang, Peng Sang, Liquan Yang, Liquan Yang, Liquan Yang   +20 more
doaj   +2 more sources