Results 41 to 50 of about 4,068 (193)

Helping proteins come in from the cold: 5 burning questions about cold-active enzymes

open access: yesBBA Advances
Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest over the past decades for their potential in various low-temperature industrial processes.
Jan Stanislaw Nowak, Daniel E. Otzen
doaj   +1 more source

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

open access: yesAdvanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch   +4 more
wiley   +1 more source

Mechanozyme: An Artificial Enzyme With a Mechanophore Framework

open access: yesAdvanced Science, EarlyView.
Mechanical destabilization of a G‐quadruplex mechanophore in a DNAzyme markedly boosts its catalytic activity, demonstrating “mechanozymes” as artificial enzymes whose functions are modulated by mechanical stress exerted by ultrasonication. This mechanozyme principle is broadly applicable to control biomolecular activities.
Jiahao Ji   +7 more
wiley   +1 more source

Nature inspired engineering: bioprospecting for novel psychrophilic enzymes from the Antarctic tunicate, Salpa thompsoni [PDF]

open access: yes, 2022
Nature-inspired engineering offers an alternative approach to solving key sustainability challenges in bioprocessing by looking to fundamental mechanisms that occur in nature for inspiration on solutions to key bioprocessing obstacles. The degradation of
Cotton, Alexander Ross
core  

Discovery of a cryptic aminoacidic triad involved in the temperature adaptation of GH1 enzymes

open access: yesThe FEBS Journal, EarlyView.
Cold‐active enzymes retain high catalytic activity at low temperatures but are characterized by thermal instability, a behavior not fully understood. Comparison between a cold‐active GH1 and its mesophilic counterparts highlights the role of a three‐residue motif (W‐M‐F) in conferring structural stability to a mesophilic GH1.
Stefania Digiovanni   +5 more
wiley   +1 more source

Evaluation of the Effects of Wax Films Formulated With Thyme and Laurel Essential Oils on Chemical and Microbiological Quality Characteristics of Rainbow Trout Fillets During Storage

open access: yesFood Science &Nutrition, Volume 14, Issue 6, June 2026.
This graphical abstract illustrates the superior preservative effects of essential oil‐enriched beeswax coatings on rainbow trout fillets compared to conventional methods. The application of these natural active coatings, particularly with 4% laurel essential oil, significantly slowed biochemical spoilage, improved textural attributes, and preserved ...
Ayşe Janseli Denizkara   +4 more
wiley   +1 more source

Study on the potential of cold-active lipases from psychrotrophic fungi for detergent formulation

open access: yesJournal of Genetic Engineering and Biotechnology, 2018
Lipases from psychrotrophic fungal isolates BPF4 and BPF6 identified as Penicilium canesense and Pseudogymnoascus roseus respectively were characterized for their compatibility towards laundry detergent.
Sanjay Sahay, Deepak Chouhan
doaj   +1 more source

Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering

open access: yesCrystals, 2020
The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called XtalController has recently been designed that allows the monitoring of crystallization assays using ...
Raphaël de Wijn   +12 more
doaj   +1 more source

Synergistic Preservation of Fresh‐Cut Kiwifruit Using Plasma‐Activated Water, Ultrasound‐Treated Water, and Modified Atmosphere Packaging: Effects on Quality, Microbial Safety, and Microstructure

open access: yesJournal of Food Process Engineering, Volume 49, Issue 6, June 2026.
Combined plasma‐activated water and ultrasound‐treated water under modified atmosphere packaging improved microbial safety and quality retention of fresh‐cut kiwifruit. Optimized treatments reduced microbial growth and preserved physicochemical and sensory attributes, demonstrating a promising nonthermal strategy for fresh produce preservation ...
Mahdi Rashvand   +4 more
wiley   +1 more source

Properties and Applications of Extremozymes from Deep-Sea Extremophilic Microorganisms: A Mini Review

open access: yesMarine Drugs, 2019
The deep sea, which is defined as sea water below a depth of 1000 m, is one of the largest biomes on the Earth, and is recognised as an extreme environment due to its range of challenging physical parameters, such as pressure, salinity, temperature ...
Min Jin   +4 more
doaj   +1 more source

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