Results 21 to 30 of about 4,068 (193)

Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation

Marine Drugs, 2021
β-galactosidases (EC 3.2.1.23) catalyze the hydrolysis of β-galactosidic bonds in oligosaccharides and, under certain conditions, transfer a sugar moiety from a glycosyl donor to an acceptor.
Marco Mangiagalli, Marina Lotti
doaj   +1 more source

CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus

Computational and Structural Biotechnology Journal, 2021
CCA-adding enzymes are highly specific RNA polymerases that add and maintain the sequence C-C-A at tRNA 3‘-ends. Recently, we could reveal that cold adaptation of such a polymerase is not only achieved at the expense of enzyme stability, but also at the ...
Raphaël de Wijn, Kévin Rollet, Felix G.M. Ernst, Karolin Wellner, Heike Betat, Mario Mörl, Claude Sauter   +6 more
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Cold-Active Enzymes and Their Potential Industrial Applications—A Review

Molecules, 2022
More than 70% of our planet is covered by extremely cold environments, nourishing a broad diversity of microbial life. Temperature is the most significant parameter that plays a key role in the distribution of microorganisms on our planet.
Burhan Hamid, Zaffar Bashir, Ali Mohd Yatoo, Fayaz Mohiddin, Neesa Majeed, Monika Bansal, Peter Poczai, Waleed Hassan Almalki, R. Z. Sayyed, Ali A. Shati, Mohammad Y. Alfaifi   +10 more
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Computational Analysis of Thermal Adaptation in Extremophilic Chitinases: The Achilles’ Heel in Protein Structure and Industrial Utilization

Molecules, 2021
Understanding protein stability is critical for the application of enzymes in biotechnological processes. The structural basis for the stability of thermally adapted chitinases has not yet been examined.
Dale L. Ang, Mubasher Zahir Hoque, Md. Abir Hossain, Gea Guerriero, Roberto Berni, Jean-Francois Hausman, Saleem A Bokhari, Wallace J. Bridge, Khawar Sohail Siddiqui   +8 more
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Structure analysis of a new psychrophilic marine protease. [PDF]

PLoS ONE, 2011
A new psychrophilic marine protease was found from a marine bacterium Flavobacterium YS-80 in the Chinese Yellow Sea. The protease is about 49 kD with an isoelectric point about 4.5.
Si-Cai Zhang, Mi Sun, Tang Li, Qi-Hai Wang, Jian-Hua Hao, Yi Han, Xiao-Jian Hu, Ming Zhou, Sheng-Xiang Lin   +8 more
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Data_Sheet_1_Discrimination of psychrophilic enzymes using machine learning algorithms with amino acid composition descriptor.docx

, 2023
IntroductionPsychrophilic enzymes are a class of macromolecules with high catalytic activity at low temperatures. Cold-active enzymes possessing eco-friendly and cost-effective properties, are of huge potential application in detergent, textiles ...
Fufeng Liu (3308805), Ailan Huang (11747052), Fuping Lu (4481230)   +2 more
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Whole genome analysis and cold adaptation strategies of Pseudomonas sivasensis W-6 isolated from the Napahai plateau wetland

Scientific Reports, 2023
Microbial communities of wetlands play key roles in the earth’s ecology and stability. To elucidate the cold adaptation mechanisms of bacteria in plateau wetlands, we conducted comparative genomic analyses of Pseudomonas sivasensis and closely related ...
Lingling Xiong, Yanmei Li, Hang Yu, Yunlin Wei, Haiyan Li, Xiuling Ji   +5 more
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Recent Progress on Methods for the Detection and Analysis of Psychrophilic Bacteria in Milk [PDF]

Shipin Kexue
Milk is an excellent medium for the growth of microorganisms, which is easily contaminated by microorganisms from the environment. Psychrophilic bacteria are the most common microorganisms in cold-stored raw milk. Moreover, psychrophilic bacteria produce
WU Jie, ZHENG Nan, WANG Jiaqi, MENG Lu
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Computer simulations explain the anomalous temperature optimum in a cold-adapted enzyme

Nature Communications, 2020
Enzymes from organisms inhabiting cold environments (psychrophiles) have adapted to catalyzing chemical reactions at near freezing temperatures. Here – using molecular dynamics simulations – the authors analyze cold adaptation of psychrophilic α-amylase ...
Jaka Sočan, Miha Purg, Johan Åqvist
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Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Frontiers in Microbiology, 2022
Raw milk is susceptible to microbial contamination during transportation and storage. Pseudomonas fluorescens producing heat-resistant enzymes have become the most common and harmful psychrophilic microorganisms in the cold chain logistics of raw milk ...
Yushan Bu, Wenjun Qiao, Zhengyuan Zhai, Tongjie Liu, Pimin Gong, Lanwei Zhang, Yanling Hao, Yanling Hao, Huaxi Yi   +8 more
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