Results 91 to 100 of about 636,686 (304)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

A geographical analysis of the role of unconventional public transport in selected rural areas of Scotland [PDF]

open access: yes, 1995
This thesis undertakes a geographical analysis of the role of unconventional public transport in selected rural areas of Scotland, with special reference to the Highland and the Borders Regions.
Robinson, Stanley Elmer
core  

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

open access: yesFEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes   +32 more
wiley   +1 more source

Managing the Accessibility on Mass Public Transit: the Case of Hong Kong

open access: yesJournal of Transport and Land Use, 2008
Public transit services (PTS) improve mobility and accessibility, and reduce car dependence. It is ideal if PTS are financially sustainable, with affordable fares and expedient quality.
Siman Tang, Hong K Lo, David Z.W. Wang
doaj  

Benchmarking public transport accessibility in Australasian cities

open access: yes, 2012
There is growing recognition by all tiers of government that transformation of the public transport system is necessary if it is to offer a real alternative to car-based travel in Australasian cities.
Curtis, Carey, Scheurer, Jan
core  

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Exploring public bus service quality in South Africa: A structural equation modelling approach

open access: yesJournal of Transport and Supply Chain Management, 2014
This study, which is a deviation from the usual practice of using SERVQUAL or an adaptedversion thereof, uses McKnight, Pagano and Paaswell’s (1986) service quality dimensions,namely reliability; extent of service; comfort; safety; and affordability ...
Ayanda M. Vilakazi, Krishna K. Govender
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Public bus transport demand elasticities in India [PDF]

open access: yes
A number of static and dynamic specifications of a log linear demand function for public transport are estimated using aggregate panel data for 22 Indian states over the period 1990 to 2001.
Filippini Massimo, Deb Kaushik
core   +1 more source

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