Results 221 to 230 of about 37,784 (244)

Expression of Adhesion Molecules in Cultured Human Pulmonary Microvascular Endothelial Cells

Microvascular Research, 1995
HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes. The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more than 20 passages (1:4 split ratio) in vitro. Cells were characterized as endothelial based upon their cobblestone morphology, the presence of factor VIII-related antigen,
J, Shen, R G, Ham, S, Karmiol
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Lipopolysaccharide impairs permeability of pulmonary microvascular endothelial cells via Connexin40

Microvascular Research, 2018
The endotoxin lipopolysaccharide (LPS)-induced pulmonary endothelial barrier disruption is a key pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the molecular mechanisms underlying LPS-impaired permeability of pulmonary microvascular endothelial cells (PMVECs) are not fully understood.
Hua-Song, Zhou, Meng, Li, Bing-Dong, Sui, Lei, Wei, Rui, Hou, Wen-Sheng, Chen, Qiang, Li, Sheng-Hui, Bi, Jin-Zhou, Zhang, Ding-Hua, Yi   +9 more
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Endothelial progenitor cells may inhibit apoptosis of pulmonary microvascular endothelial cells: new insights into cell therapy for pulmonary arterial hypertension

Cytotherapy, 2009
Endothelial apoptosis underlies the pathophysiology of pulmonary arterial hypertension (PAH). Some factors/cytokines released by endothelial progenitor cells (EPC) have been revealed as potent inhibitors of apoptosis. The aim of this study was to investigate the effects of EPC on pulmonary microvascular endothelial cell (PMVEC) survival with the PAH ...
Liang, Xia, Guo-Sheng, Fu, Jin-Xiu, Yang, Fu-Rong, Zhang, Xing-Xiang, Wang   +4 more
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Microparticle Delivery of Cyclic AMP to Pulmonary Microvascular Endothelial Cells

The FASEB Journal, 2017
Microparticles (MPs) are important for the exchange of information between cells. MPs are extracellular vesicles that range in size from 0.1 to 1 μm in diameter. They are constitutively released from many cell types and are thought to play a role in vascular homeostasis.
April K Scruggs, Silas J Leavesley, Naga S Annamdevula, Thomas C Rich, Natalie N Bauer   +4 more
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[A method for primary culture of pulmonary microvascular endothelial cells].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2014
To explore a simple and practical method of primarily culturing rat pulmonary microvascular endothelial cells (PMECs) in vitro, and observe the cell growth status and identify the PMECs.Wistar rats (n=40, aged 4-5 weeks) were sacrificed to take the lung tissue.
Jiantao, Jia, Huiying, Zhang, Limin, Wang, Zhongfu, Zhao, Mingshe, Liu, Lina, Lai, Yanzhen, Lu   +6 more
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Cyclic GMP accumulation in pulmonary microvascular endothelial cells measured by intact cell prelabeling

Life Sciences, 1997
Cyclic GMP accumulation in cultured rat pulmonary microvascular endothelial cells (RPMVEC) was studied with a new prelabeling method developed using intact platelets and smooth muscle cells (1). [3H]-hypoxanthine was used to radiolabel the cellular guanine nucleotide pool. Neutral alumina and Dowex-50 double column chromatography was used to purify and
P D, Reynolds, S J, Strada, W J, Thompson   +2 more
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The role of aging on endothelial cell-cell junctions and pulmonary microvascular permeability

Abstract Lung injury leads to pulmonary microvascular endothelial cell (PMVEC) damage, disruption of cell-cell junctions, and increased permeability. Previously, we demonstrated in a mechanical ventilation-induced model of lung injury that aging exacerbated pulmonary microvascular permeability.
Aminmohamed Manji, Lefeng Wang, Cynthia Pape, Sanjay Mehta, Preya Patel, Samuel-Caleb Yeung, Eric K. Patterson, Antoine Dufour, Daniel Young, Ruud A.W. Veldhuizen, Sean E. Gill   +10 more
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[Investigation on culture of rat pulmonary microvascular endothelial cells and their viscoelasticity].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2003
It is the infent of this study to establish a simple method for cultivation of rat pulmonary microvascular endothelial cells(PMVECs) and investigate the viscoelasticity of PMVECs. First, we obtained rat's peripheral pulmonary tissue, which then was cut into small pieces and cultured with 3 ml DMEM containing 20% bovine calf serum, 90 U/ml heparin, 4 ...
Faqi, Li, Ruifang, Yang, Qiping, Huang, Jiang, Qin, Shaoxi, Cai, Yunpeng, Wu   +5 more
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[Improving Primary Culture of Pulmonary Microvascular Endothelial Cells of Rats].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2018
To improve the culturing method of pulmonary microvascular endothelial cells (PMEVCs) of SD rats.The culturing processes in regard to obtaining peripheral lung tissue, attaching tissue block,preparing medium and subculturing were modified.These included an injection of heparin sodium before anesthesia, abdominal bleeding, opening of chest when ...
Ling, Jiang, Yuan-Dong, Hu, Fei-Fei, Xu, Ting-Hua, Wang   +3 more
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[Protection Mechanism of Alpinetin on Pulmonary Microvascular Endothelial Cells Injury].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, 2019
Objective To explore the protection mechanisms about Alpinetin to acute human pulmonary microvascular endothelial cells ( HPMECs ) injury. Methods Different concentration of LPS (0. 01,0. 1 ,1. ,10 mg/L) was applied to the HPMECs to induce acute HPMECs injury. The HPMECs mod- els (n =3) were intervened by Alpinetin(40,80,160,320 mg/L) .
Zhen-Ran, Wang, Yang, Li, Bo, Tang
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