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Genetic Variability of Purine Nucleoside Phosphorylase in the Mouse
1984The inherited human deficiency of purine nucleoside phosphorylase is associated primarily with cellular immune dysfunction1. We have begun to screen for quantitative activity variants of purine nucleoside phosphorylase in Mus musculus in attempts to establish an animal model for the enzyme deficiencies associated with immunodeficiency disease.
Marcia J. Sparling +3 more
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Chapter 3. Purine Nucleoside Phosphorylase
2007Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2′-deoxypurine ribonucleosides to the free base, and ribose 1-phosphate or 2′-deoxyribose 1-phosphate, respectively.1–3 PNPs are found in most prokaryotic and eukaryotic organisms where the en...
Steven E. Ealick, Yang Zhang
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FISH-MUSCLE PURINE AND PYRIMIDINE NUCLEOSIDE PHOSPHORYLASES
Canadian Journal of Biochemistry, 1967Three purine nucleoside phosphorylase preparations (isoenzymes) were obtained by ammonium sulfate fractionation and DEAE-cellulose chromatography of aqueous extracts of lingcod muscle. Dialysis, adsorption on alumina Cγ, and elution with 0.4 M phosphate buffer yielded further purification.
Joan E. Roy, H. L. A. Tarr
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Plasma purine nucleoside phosphorylase in cancer patients
Clinica Chimica Acta, 2004Purine nucleoside phosphorylase (PNP) is the purine salvage enzyme that converts guanosine to guanine and inosine to hypoxanthine.279 samples from patients with differing cancers were collected during treatment at both pre- and post-dose stages for plasma PNP activity and compared with a normal population.Normal plasma PNP activity was found to be 3.2+/
Russell P. Newton +2 more
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[73] Chinese hamster purine nucleoside phosphorylase
1978Publisher Summary This chapter describes the purification procedure of nucleoside phosphorylase enzyme from Chinese hamster V79 tissue culture cells. Purine nucleoside phosphorylase (purine nucleoside: orthophosphate ribosyltransferase) in eukaryotes catalyzes the reversible conversion among the purine bases, hypoxanthine and guanine, and their ...
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Inhibition of human purine nucleoside phosphorylase by acyclic nucleosides and nucleotides
Biochemical Pharmacology, 1987In an effort to develop more potent inhibitors of human purine nucleoside phosphorylase (PNP) as immunosuppressive and cancer chemotherapeutic agents, the affinity of the erythrocytic enzyme for 30 acyclic nucleosides, nucleotides and related compounds was determined.
Robert E. Parks +8 more
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Purine nucleoside synthesis: an efficient method employing nucleoside phosphorylases
Biochemistry, 1981An improved method for the enzymatic synthesis of purine nucleosides is described. Pyrimidine nucleosides were used as pentosyl donors and two phosphorylases were used as catalysts. One of the enzymes, either uridine phosphorylase (Urd Pase) or thymidine phosphorylase (dThd Pase), catalyzed the phosphorolysis of the pentosyl donor.
Joel Van Tuttle +2 more
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Purine Nucleoside Phosphorylase of Bovine Liver Mitochondria
1991Purine nucleoside phosphorylase (PNPase, purine nucleoside: orthophosphate ribosyltransferase E.C. 2.4.2.1) in mammals reversibly catalyzes the phosphorolysis of the purine ribonucleosides (deoxy)inosine and (deoxy)guanosine to (deoxy)ribose-l-phosphate and the respective base.
Roger A. Lewis, Robert K. Haag
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