Synthesis of Substituted 1,2,4-Triazole-3-Thione Nucleosides Using E. coli Purine Nucleoside Phosphorylase [PDF]
Biomolecules1,2,4-Triazole derivatives have a wide range of biological activities. The most well-known drug that contains 1,2,4-triazole as part of its structure is the nucleoside analogue ribavirin, an antiviral drug. Finding new nucleosides based on 1,2,4-triazole
Ilya V. Fateev +22 more
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Evaluating antitumor activity of Escherichia coli purine nucleoside phosphorylase against head and neck patient‐derived xenografts [PDF]
Cancer Reports, 2023 Background Purine nucleoside phosphorylase (PNP) gene transfer represents a promising approach to treatment of head and neck malignancies. We tested recombinant adenovirus already in phase I/II clinical testing and leading‐edge patient‐derived xenografts
Regina Rab +10 more
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The Characterization of the Purine Nucleoside Phosphorylase from Agaricus bisporus and Its Potential Application in Reducing Purine Content in Beer [PDF]
Journal of FungiBeer, the most popular alcoholic beverage, poses health risks for individuals with gout and hyperuricemia due to its high purine content. Herein, we identified a novel purine nucleoside phosphorylase (AbPNP) from the edible mushroom Agaricus bisporus and
Jun Liu, Jian Lu
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Trimeric Architecture Ensures the Stability and Biological Activity of the Calf Purine Nucleoside Phosphorylase: In Silico and In Vitro Studies of Monomeric and Trimeric Forms of the Enzyme. [PDF]
Int J Mol Sci, 2023 Dyzma A +7 more
europepmc +3 more sources
Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance Enzymes achieve catalytic efficiency by optimizing contacts between reactants and catalytic site amino acids. The transition state forms rarely, with a lifetime of a few femtoseconds.
Harijan RK +4 more
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Purine nucleoside phosphorylase as a target for the treatment of interstitial cystitis/bladder pain syndrome with and without Hunner lesions [PDF]
Scientific ReportsChronic visceral pain disorders, such as interstitial cystitis/bladder pain syndrome (IC/BPS), are difficult to treat, and therapies are limited in number and efficacy.
Lori A. Birder +6 more
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Nature Communications, 2015 The study of biomolecular interactions between a drug and its biological target is of paramount importance for the design of novel bioactive compounds.
Sergio Decherchi +2 more
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Radical Dehalogenation and Purine Nucleoside Phosphorylase E. coli: How Does an Admixture of 2′,3′-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis [PDF]
Biomolecules, 2021 During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3′-deoxyinosine catalyzed by E. coli purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered.
Alexey L. Kayushin +12 more
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Crystallographic snapshots of ligand binding to hexameric purine nucleoside phosphorylase and kinetic studies give insight into the mechanism of catalysis. [PDF]
Sci Rep, 2018 Purine nucleoside phosphorylase (PNP) catalyses the cleavage of the glycosidic bond of purine nucleosides using phosphate instead of water as a second substrate.
Štefanić Z +5 more
europepmc +2 more sources